Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation

Shape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific biophysical signaling by which changes in cell shape contribute to cell cycle regul...

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Autores: Roca-Cusachs Soulere, Pere, Alcaraz Casademunt, Jordi, Sunyer, Raimon, Samitier i Martí, Josep, Farré Ventura, Ramon, Navajas Navarro, Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/25802
Acceso en línea:https://hdl.handle.net/2445/25802
Access Level:acceso abierto
Palabra clave:Cèl·lules epitelials
Regulació cel·lular
Cicle cel·lular
Epithelial cells
Cellular control mechanisms
Cell cycle
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spelling Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferationRoca-Cusachs Soulere, PereAlcaraz Casademunt, JordiSunyer, RaimonSamitier i Martí, JosepFarré Ventura, RamonNavajas Navarro, DanielCèl·lules epitelialsRegulació cel·lularCicle cel·lularEpithelial cellsCellular control mechanismsCell cycleShape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific biophysical signaling by which changes in cell shape contribute to cell cycle regulation remains poorly understood. Here, we describe our study of the roles of nuclear volume and cytoskeletal mechanics in mediating shape control of proliferation in single endothelial cells. Micropatterned adhesive islands were used to independently control cell spreading and elongation. We show that, irrespective of elongation, nuclear volume and apparent chromatin decondensation of cells in G1 systematically increased with cell spreading and highly correlated with DNA synthesis (percent of cells in the S phase). In contrast, cell elongation dramatically affected the organization of the actin cytoskeleton, markedly reduced both cytoskeletal stiffness (measured dorsally with atomic force microscopy) and contractility (measured ventrally with traction microscopy), and increased mechanical anisotropy, without affecting either DNA synthesis or nuclear volume. Our results reveal that the nuclear volume in G1 is predictive of the proliferative status of single endothelial cells within a population, whereas cell stiffness and contractility are not. These findings show that the effects of cell mechanics in shape control of proliferation are far more complex than a linear or straightforward relationship. Our data are consistent with a mechanism by which spreading of cells in G1 partially enhances proliferation by inducing nuclear swelling and decreasing chromatin condensation, thereby rendering DNA more accessible to the replication machinery.Biophysical Society2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/25802Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1529/biophysj.107.116863Biophysical Journal, 2008, vol. 94, núm. 12, p. 4984-4995http://dx.doi.org/10.1529/biophysj.107.116863(c) The Biophysical Society, 2008info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/258022026-05-27T06:46:51Z
dc.title.none.fl_str_mv Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
title Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
spellingShingle Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
Roca-Cusachs Soulere, Pere
Cèl·lules epitelials
Regulació cel·lular
Cicle cel·lular
Epithelial cells
Cellular control mechanisms
Cell cycle
title_short Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
title_full Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
title_fullStr Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
title_full_unstemmed Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
title_sort Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
dc.creator.none.fl_str_mv Roca-Cusachs Soulere, Pere
Alcaraz Casademunt, Jordi
Sunyer, Raimon
Samitier i Martí, Josep
Farré Ventura, Ramon
Navajas Navarro, Daniel
author Roca-Cusachs Soulere, Pere
author_facet Roca-Cusachs Soulere, Pere
Alcaraz Casademunt, Jordi
Sunyer, Raimon
Samitier i Martí, Josep
Farré Ventura, Ramon
Navajas Navarro, Daniel
author_role author
author2 Alcaraz Casademunt, Jordi
Sunyer, Raimon
Samitier i Martí, Josep
Farré Ventura, Ramon
Navajas Navarro, Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules epitelials
Regulació cel·lular
Cicle cel·lular
Epithelial cells
Cellular control mechanisms
Cell cycle
topic Cèl·lules epitelials
Regulació cel·lular
Cicle cel·lular
Epithelial cells
Cellular control mechanisms
Cell cycle
description Shape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific biophysical signaling by which changes in cell shape contribute to cell cycle regulation remains poorly understood. Here, we describe our study of the roles of nuclear volume and cytoskeletal mechanics in mediating shape control of proliferation in single endothelial cells. Micropatterned adhesive islands were used to independently control cell spreading and elongation. We show that, irrespective of elongation, nuclear volume and apparent chromatin decondensation of cells in G1 systematically increased with cell spreading and highly correlated with DNA synthesis (percent of cells in the S phase). In contrast, cell elongation dramatically affected the organization of the actin cytoskeleton, markedly reduced both cytoskeletal stiffness (measured dorsally with atomic force microscopy) and contractility (measured ventrally with traction microscopy), and increased mechanical anisotropy, without affecting either DNA synthesis or nuclear volume. Our results reveal that the nuclear volume in G1 is predictive of the proliferative status of single endothelial cells within a population, whereas cell stiffness and contractility are not. These findings show that the effects of cell mechanics in shape control of proliferation are far more complex than a linear or straightforward relationship. Our data are consistent with a mechanism by which spreading of cells in G1 partially enhances proliferation by inducing nuclear swelling and decreasing chromatin condensation, thereby rendering DNA more accessible to the replication machinery.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/25802
url https://hdl.handle.net/2445/25802
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1529/biophysj.107.116863
Biophysical Journal, 2008, vol. 94, núm. 12, p. 4984-4995
http://dx.doi.org/10.1529/biophysj.107.116863
dc.rights.none.fl_str_mv (c) The Biophysical Society, 2008
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) The Biophysical Society, 2008
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biophysical Society
publisher.none.fl_str_mv Biophysical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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