Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

[EN] Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequenci...

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Detalles Bibliográficos
Autores: Beier, Sebastian 1983-, Himmelbach, Axel, Colmsee, Christian, Zhang, Xiao-Qi, Barrero, Roberto A., Zhang, Qisen, Li, Lin, Bayer, Micha, Bolser, Daniel, Taudien, Stefan, Groth, Marco, Felder, Marius 1965-, Hastie, Alex, Šimková, Hana, Staňková, Helena, Vrána, Jan, Chan, Saki, Muñoz Amatriaín, María, Ounit, Rachid, Wanamaker, Steve I., Schmutzer, Thomas 1982-, Aliyeva-Schnorr, Lala, Grasso, Stefano, Tanskanen, Jaakko, Sampath, Dharanya, Heavens, Darren, Cao, Sujie, Chapman, Brett, Dai, Fei, Han, Yong, Li, Hua, Li, Xuan, Lin, Chongyun, McCooke, John K., Tan, Cong, Wang, Songbo, Yin, Shuya, Zhou, Gaofeng, Poland, Jesse A., Bellgard, Matthew I., Houben, Andreas, Doležel, Jaroslav, Ayling, Sarah, Lonardi, Stefano, Langridge, Peter, Muehlbauer, Gary J., Kersey, Paul, Clark, Matthew D., Caccamo, Mario, Schulman, Alan H., Platzer, Matthias, Close, Timothy James, Hansson, Mats, Zhang, Guoping, Braumann, Ilka, Li, Chengdao, Waugh, Robbie, Scholz, Uwe 1963-, Stein, Nils, Mascher, Martin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24788
Acceso en línea:https://www.nature.com/articles/sdata201744
https://hdl.handle.net/10612/24788
Access Level:acceso abierto
Palabra clave:Biología
Genética
Barley (Hordeum vulgare L.)
Genome sequence
DNA sequencing
Genome assembly algorithms
Plant genetics
Barley genome explorer (BARLEX)
2417.14 Genética Vegetal
Descripción
Sumario:[EN] Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. ‘Morex’ was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX)

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