Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species

In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius),...

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Autores: Vallecillos Quijada, Antonio, María Dolores Pedrero, Emilio, Villa Navarro, Javier, Rueda González, Francisco Miguel, Carrillo, José, Ramis Vidal, Guillermo, Soula, Mohamed, Afonso López, Juan Manuel, Armero Ibáñez, Eva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad Politécnica de Cartagena(UPCT)
Repositorio:Repositorio Digital UPCT
OAI Identifier:oai:repositorio.upct.es:10317/11926
Acceso en línea:http://hdl.handle.net/10317/11926
https://www.mdpi.com/2410-3888/7/3/117
Access Level:acceso abierto
Palabra clave:Meagre (Argyrosomus regius)
Microsatellites
Parental assignment
PCR
Population
Producción Animal
3104 Producción Animal
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spelling Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture speciesVallecillos Quijada, AntonioMaría Dolores Pedrero, EmilioVilla Navarro, JavierRueda González, Francisco MiguelCarrillo, JoséRamis Vidal, GuillermoSoula, MohamedAfonso López, Juan ManuelArmero Ibáñez, EvaMeagre (Argyrosomus regius)MicrosatellitesParental assignmentPCRPopulationProducción Animal3104 Producción AnimalIn this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups.This research was funded by Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia through the project “Mejora de la competitividad del sector de la corvina a través de la selección genética (GENECOR, 21002/PI/18)” in the call for grants for projects for the development of scientific and technical research by competitive groups, included in the Regional Program for the Promotion of Scientific and Technical Research (Action Plan 2018). A.V. was funded by a pre-doctora research fellowship (20716/FPI/18. Fundación Séneca. Cofinanciado por grupo Avramar S.L. Región de Murcia (Spain)). The Avramar S.L. group provided financial support and the use of its facilities for this study.MDPIFundación SénecaAvramar S.L. Región de Murcia (Spain)202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10317/11926https://www.mdpi.com/2410-3888/7/3/117reponame:Repositorio Digital UPCTinstname:Universidad Politécnica de Cartagena(UPCT)Ingléshttp://hdl.handle.net/10317/11382Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.upct.es:10317/119262026-05-15T06:39:02Z
dc.title.none.fl_str_mv Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
title Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
spellingShingle Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
Vallecillos Quijada, Antonio
Meagre (Argyrosomus regius)
Microsatellites
Parental assignment
PCR
Population
Producción Animal
3104 Producción Animal
title_short Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
title_full Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
title_fullStr Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
title_full_unstemmed Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
title_sort Development of the first microsatellite multiplex PCR panel for meagre (Argyrosomus regius), a commercial aquaculture species
dc.creator.none.fl_str_mv Vallecillos Quijada, Antonio
María Dolores Pedrero, Emilio
Villa Navarro, Javier
Rueda González, Francisco Miguel
Carrillo, José
Ramis Vidal, Guillermo
Soula, Mohamed
Afonso López, Juan Manuel
Armero Ibáñez, Eva
author Vallecillos Quijada, Antonio
author_facet Vallecillos Quijada, Antonio
María Dolores Pedrero, Emilio
Villa Navarro, Javier
Rueda González, Francisco Miguel
Carrillo, José
Ramis Vidal, Guillermo
Soula, Mohamed
Afonso López, Juan Manuel
Armero Ibáñez, Eva
author_role author
author2 María Dolores Pedrero, Emilio
Villa Navarro, Javier
Rueda González, Francisco Miguel
Carrillo, José
Ramis Vidal, Guillermo
Soula, Mohamed
Afonso López, Juan Manuel
Armero Ibáñez, Eva
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundación Séneca
Avramar S.L. Región de Murcia (Spain)
dc.subject.none.fl_str_mv Meagre (Argyrosomus regius)
Microsatellites
Parental assignment
PCR
Population
Producción Animal
3104 Producción Animal
topic Meagre (Argyrosomus regius)
Microsatellites
Parental assignment
PCR
Population
Producción Animal
3104 Producción Animal
description In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10317/11926
https://www.mdpi.com/2410-3888/7/3/117
url http://hdl.handle.net/10317/11926
https://www.mdpi.com/2410-3888/7/3/117
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://hdl.handle.net/10317/11382
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital UPCT
instname:Universidad Politécnica de Cartagena(UPCT)
instname_str Universidad Politécnica de Cartagena(UPCT)
reponame_str Repositorio Digital UPCT
collection Repositorio Digital UPCT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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