Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.

In the context of measles elimination, the identification of recent infections is important for clinical laboratories. Serological diagnosis is achieved by detecting specific IgG and IgM. Recently an automated chemiluminescent immunoassay (CLIA) (Liaison, DiaSorin, Italy) has been used to quantify t...

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Detalles Bibliográficos
Autores: Sanz, Juan C, De Ory, Fernando de, Minguito, Teodora, Balfagon, Pilar
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/10446
Acceso en línea:http://hdl.handle.net/20.500.12105/10446
Access Level:acceso abierto
Palabra clave:Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Immunoglobulin G
Immunoglobulin M
Italy
Measles
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spelling Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.Sanz, Juan CDe Ory, Fernando deMinguito, TeodoraBalfagon, PilarEnzyme-Linked Immunosorbent AssayHumansImmunoassayImmunoglobulin GImmunoglobulin MItalyMeaslesIn the context of measles elimination, the identification of recent infections is important for clinical laboratories. Serological diagnosis is achieved by detecting specific IgG and IgM. Recently an automated chemiluminescent immunoassay (CLIA) (Liaison, DiaSorin, Italy) has been used to quantify the measles antibody. The aim of this study was to compare this assay with Enzygnost ELISA (Siemens, Germany), with final classification of discrepancies by indirect immunofluorescence (Euroimmun, Germany). For measles IgM, 204 sera were analyzed: 50 IgM-positive, 104 IgM-negative/IgG-positive, and 50 from other viral infections (B19V, rubella, mumps, CMV, and EBV). For the measles IgG assay, 162 samples were tested: 106 were positive and 56 were negative. For measles IgM, the sensitivity and specificity of CLIA against ELISA were 94% (95% CI: 83.2-98.6) and 100% (95% CI: 97.1-100), respectively; the corrected figures after the final classification of discrepancies were 100% (95% CI: 91.0-100) and 99.4% (95% CI: 96.1-100), respectively. In relation to IgG, the sensitivity and specificity of CLIA against ELISA were, respectively, 97.2% (95% CI: 91.7-99.4) and 92.9% (95% CI: 82.5-97.7), and 95.5% (95% CI: 89.5-98.3) and 100% (95% CI: 91.8-100) after the final classification. CLIA showed excellent sensitivity and specificity in detecting measles IgG and IgM antibodies, eliminating the need to aliquot specimens before carrying out the assay.WileyInstituto de Salud Carlos III20202020-06-1620152015-08-0120152015-08-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/10446reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/104462026-06-12T12:43:37Z
dc.title.none.fl_str_mv Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
title Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
spellingShingle Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
Sanz, Juan C
Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Immunoglobulin G
Immunoglobulin M
Italy
Measles
title_short Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
title_full Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
title_fullStr Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
title_full_unstemmed Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
title_sort Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.
dc.creator.none.fl_str_mv Sanz, Juan C
De Ory, Fernando de
Minguito, Teodora
Balfagon, Pilar
author Sanz, Juan C
author_facet Sanz, Juan C
De Ory, Fernando de
Minguito, Teodora
Balfagon, Pilar
author_role author
author2 De Ory, Fernando de
Minguito, Teodora
Balfagon, Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Immunoglobulin G
Immunoglobulin M
Italy
Measles
topic Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Immunoglobulin G
Immunoglobulin M
Italy
Measles
description In the context of measles elimination, the identification of recent infections is important for clinical laboratories. Serological diagnosis is achieved by detecting specific IgG and IgM. Recently an automated chemiluminescent immunoassay (CLIA) (Liaison, DiaSorin, Italy) has been used to quantify the measles antibody. The aim of this study was to compare this assay with Enzygnost ELISA (Siemens, Germany), with final classification of discrepancies by indirect immunofluorescence (Euroimmun, Germany). For measles IgM, 204 sera were analyzed: 50 IgM-positive, 104 IgM-negative/IgG-positive, and 50 from other viral infections (B19V, rubella, mumps, CMV, and EBV). For the measles IgG assay, 162 samples were tested: 106 were positive and 56 were negative. For measles IgM, the sensitivity and specificity of CLIA against ELISA were 94% (95% CI: 83.2-98.6) and 100% (95% CI: 97.1-100), respectively; the corrected figures after the final classification of discrepancies were 100% (95% CI: 91.0-100) and 99.4% (95% CI: 96.1-100), respectively. In relation to IgG, the sensitivity and specificity of CLIA against ELISA were, respectively, 97.2% (95% CI: 91.7-99.4) and 92.9% (95% CI: 82.5-97.7), and 95.5% (95% CI: 89.5-98.3) and 100% (95% CI: 91.8-100) after the final classification. CLIA showed excellent sensitivity and specificity in detecting measles IgG and IgM antibodies, eliminating the need to aliquot specimens before carrying out the assay.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-08-01
2015
2015-08-01
2020
2020-06-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/10446
url http://hdl.handle.net/20.500.12105/10446
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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