Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration

Figure S1 Relative gene expression of ALP, BSPII, and RUNX2 genes in hBM-MSCs cultured for 3 days in osteogenic condition and COLII gene for 3 days in chondrogenic condition. *** p < 0.01 as compared to PCL, Figure S2 Staining with Alkaline Phosphatase after 7 days of culture of hBM-MSCs on the t...

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Autores: Smaida, Rana, Pijnenburg, Luc, Irusta, Silvia, Himawan, Erico, Mendoza, Gracia, Harmouch, Ezeddine, Idoux-Gillet, Ysia, Kuchler-Bopp, Sabine, Benkirane-Jessel, Nadia, Hua, Guoqiang
Tipo de recurso: conjunto de datos
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/368865
Acceso en línea:http://hdl.handle.net/10261/368865
Access Level:acceso abierto
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spelling Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regenerationSmaida, RanaPijnenburg, LucIrusta, SilviaHimawan, EricoMendoza, GraciaHarmouch, EzeddineIdoux-Gillet, YsiaKuchler-Bopp, SabineBenkirane-Jessel, NadiaHua, GuoqiangFigure S1 Relative gene expression of ALP, BSPII, and RUNX2 genes in hBM-MSCs cultured for 3 days in osteogenic condition and COLII gene for 3 days in chondrogenic condition. *** p < 0.01 as compared to PCL, Figure S2 Staining with Alkaline Phosphatase after 7 days of culture of hBM-MSCs on the two types of scaffolds (PCL and PCL-HAp) in an osteogenic medium. Bars represent 4 mm in (A,C) and 50 µm in (B,D), Figure S3 In vivo subcutaneous implantation of a sterilized scaffold for two weeks. (A,B) Macroscopic analyses showing the good integration and vascularization of the implant, (C,D) Haematoxylin-Eosin (HE) staining, yellow zone: implanted membrane; red zones: blood vessels; blue zones: immune cell aggregates; and white asterisk: fibrocytes, Figure S4 Haematoxylin-Eosin (HE) staining (a–h) and histological staining with Alizarin Red S (i–p) for PCL, PCL-HAp, PCL-HA, and PCL-HAp-HA scaffolds subcutaneously implanted for 4 weeks without cells in ICR mice. S4-1 in (a,e,i,m) for the PCL scaffold S4-2 in (b,f,j,n) for the PCL-HAp scaffold; S4-3 in (c,g,k,o) for the PCL-HA scaffold; and S4-4 in (d,h,l,p) for the PCL-HAp-HA scaffold. Black arrowheads indicate vascularization. Black squares indicate the enlarged areas.Peer reviewedMultidisciplinary Digital Publishing Institute202420242020info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/pdfhttp://hdl.handle.net/10261/368865reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSmaida, Rana; Pijnenburg, Luc; Irusta, Silvia; Himawan, Erico; Mendoza, Gracia; Harmouch, Ezeddine; Idoux-Gillet, Ysia; Kuchler-Bopp, Sabine; Benkirane-Jessel, Nadia; Hua, Guoqiang. Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration. https://doi.org/10.3390/ma13143087. http://hdl.handle.net/10261/368864Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3688652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
title Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
spellingShingle Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
Smaida, Rana
title_short Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
title_full Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
title_fullStr Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
title_full_unstemmed Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
title_sort Supplementary Information: Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration
dc.creator.none.fl_str_mv Smaida, Rana
Pijnenburg, Luc
Irusta, Silvia
Himawan, Erico
Mendoza, Gracia
Harmouch, Ezeddine
Idoux-Gillet, Ysia
Kuchler-Bopp, Sabine
Benkirane-Jessel, Nadia
Hua, Guoqiang
author Smaida, Rana
author_facet Smaida, Rana
Pijnenburg, Luc
Irusta, Silvia
Himawan, Erico
Mendoza, Gracia
Harmouch, Ezeddine
Idoux-Gillet, Ysia
Kuchler-Bopp, Sabine
Benkirane-Jessel, Nadia
Hua, Guoqiang
author_role author
author2 Pijnenburg, Luc
Irusta, Silvia
Himawan, Erico
Mendoza, Gracia
Harmouch, Ezeddine
Idoux-Gillet, Ysia
Kuchler-Bopp, Sabine
Benkirane-Jessel, Nadia
Hua, Guoqiang
author2_role author
author
author
author
author
author
author
author
author
description Figure S1 Relative gene expression of ALP, BSPII, and RUNX2 genes in hBM-MSCs cultured for 3 days in osteogenic condition and COLII gene for 3 days in chondrogenic condition. *** p < 0.01 as compared to PCL, Figure S2 Staining with Alkaline Phosphatase after 7 days of culture of hBM-MSCs on the two types of scaffolds (PCL and PCL-HAp) in an osteogenic medium. Bars represent 4 mm in (A,C) and 50 µm in (B,D), Figure S3 In vivo subcutaneous implantation of a sterilized scaffold for two weeks. (A,B) Macroscopic analyses showing the good integration and vascularization of the implant, (C,D) Haematoxylin-Eosin (HE) staining, yellow zone: implanted membrane; red zones: blood vessels; blue zones: immune cell aggregates; and white asterisk: fibrocytes, Figure S4 Haematoxylin-Eosin (HE) staining (a–h) and histological staining with Alizarin Red S (i–p) for PCL, PCL-HAp, PCL-HA, and PCL-HAp-HA scaffolds subcutaneously implanted for 4 weeks without cells in ICR mice. S4-1 in (a,e,i,m) for the PCL scaffold S4-2 in (b,f,j,n) for the PCL-HAp scaffold; S4-3 in (c,g,k,o) for the PCL-HA scaffold; and S4-4 in (d,h,l,p) for the PCL-HAp-HA scaffold. Black arrowheads indicate vascularization. Black squares indicate the enlarged areas.
publishDate 2020
dc.date.none.fl_str_mv 2020
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/368865
url http://hdl.handle.net/10261/368865
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Smaida, Rana; Pijnenburg, Luc; Irusta, Silvia; Himawan, Erico; Mendoza, Gracia; Harmouch, Ezeddine; Idoux-Gillet, Ysia; Kuchler-Bopp, Sabine; Benkirane-Jessel, Nadia; Hua, Guoqiang. Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration. https://doi.org/10.3390/ma13143087. http://hdl.handle.net/10261/368864
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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