Retention of paternal DNA methylome in the developing zebrafish germline

Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish...

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Autores: Skvortsova, Ksenia, Tarbashevich, Katsiaryna, Stehling, Martin, Lister, Ryan, Irimia Martínez, Manuel, Raz, Erez, Bogdanovic, Ozren
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/42360
Acesso em linha:http://hdl.handle.net/10230/42360
http://dx.doi.org/10.1038/s41467-019-10895-6
Access Level:acceso abierto
Palavra-chave:DNA methylation
Epigenetic memory
Epigenetics
Epigenomics
Germline development
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spelling Retention of paternal DNA methylome in the developing zebrafish germlineSkvortsova, KseniaTarbashevich, KatsiarynaStehling, MartinLister, RyanIrimia Martínez, ManuelRaz, ErezBogdanovic, OzrenDNA methylationEpigenetic memoryEpigeneticsEpigenomicsGermline developmentTwo waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.Nature Research201920192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/42360http://dx.doi.org/10.1038/s41467-019-10895-6reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésNature Communications. 2019;10(1):3054© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/423602026-05-29T05:05:01Z
dc.title.none.fl_str_mv Retention of paternal DNA methylome in the developing zebrafish germline
title Retention of paternal DNA methylome in the developing zebrafish germline
spellingShingle Retention of paternal DNA methylome in the developing zebrafish germline
Skvortsova, Ksenia
DNA methylation
Epigenetic memory
Epigenetics
Epigenomics
Germline development
title_short Retention of paternal DNA methylome in the developing zebrafish germline
title_full Retention of paternal DNA methylome in the developing zebrafish germline
title_fullStr Retention of paternal DNA methylome in the developing zebrafish germline
title_full_unstemmed Retention of paternal DNA methylome in the developing zebrafish germline
title_sort Retention of paternal DNA methylome in the developing zebrafish germline
dc.creator.none.fl_str_mv Skvortsova, Ksenia
Tarbashevich, Katsiaryna
Stehling, Martin
Lister, Ryan
Irimia Martínez, Manuel
Raz, Erez
Bogdanovic, Ozren
author Skvortsova, Ksenia
author_facet Skvortsova, Ksenia
Tarbashevich, Katsiaryna
Stehling, Martin
Lister, Ryan
Irimia Martínez, Manuel
Raz, Erez
Bogdanovic, Ozren
author_role author
author2 Tarbashevich, Katsiaryna
Stehling, Martin
Lister, Ryan
Irimia Martínez, Manuel
Raz, Erez
Bogdanovic, Ozren
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DNA methylation
Epigenetic memory
Epigenetics
Epigenomics
Germline development
topic DNA methylation
Epigenetic memory
Epigenetics
Epigenomics
Germline development
description Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/42360
http://dx.doi.org/10.1038/s41467-019-10895-6
url http://hdl.handle.net/10230/42360
http://dx.doi.org/10.1038/s41467-019-10895-6
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nature Communications. 2019;10(1):3054
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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