Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish

B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recom...

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Detalles Bibliográficos
Autores: Perdiguero Jiménez, Pedro, Morel, Esther, Díaz-Rosales, Patricia, Tafalla, Carolina
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/96623
Acceso en línea:https://hdl.handle.net/20.500.14352/96623
Access Level:acceso abierto
Palabra clave:577.112
597
Bioquímica (Biología)
31 Ciencias Agrarias
2403 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/96623
network_acronym_str ES
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repository_id_str
spelling Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fishPerdiguero Jiménez, PedroMorel, EstherDíaz-Rosales, PatriciaTafalla, Carolina577.112597Bioquímica (Biología)31 Ciencias Agrarias2403 BioquímicaB cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VHDJH and VLJL genes. To ensure that one B cell only expresses one antibody, VHDJH rearrangement occurs only in one IgH locus (allelic exclusion), whereas VLJL rearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct CL genes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VLJLCL genes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.ElsevierUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/96623reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/966232026-06-02T12:44:21Z
dc.title.none.fl_str_mv Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
title Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
spellingShingle Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
Perdiguero Jiménez, Pedro
577.112
597
Bioquímica (Biología)
31 Ciencias Agrarias
2403 Bioquímica
title_short Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
title_full Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
title_fullStr Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
title_full_unstemmed Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
title_sort Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish
dc.creator.none.fl_str_mv Perdiguero Jiménez, Pedro
Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
author Perdiguero Jiménez, Pedro
author_facet Perdiguero Jiménez, Pedro
Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
author_role author
author2 Morel, Esther
Díaz-Rosales, Patricia
Tafalla, Carolina
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.112
597
Bioquímica (Biología)
31 Ciencias Agrarias
2403 Bioquímica
topic 577.112
597
Bioquímica (Biología)
31 Ciencias Agrarias
2403 Bioquímica
description B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VHDJH and VLJL genes. To ensure that one B cell only expresses one antibody, VHDJH rearrangement occurs only in one IgH locus (allelic exclusion), whereas VLJL rearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct CL genes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VLJLCL genes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/96623
url https://hdl.handle.net/20.500.14352/96623
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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