Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas

Nitric oxide (NO) and somatostatin (SS) are two important mediators of the exocrine and endocrine pancreas, exerting opposite effects on this organ. There is strong evidence suggesting an interaction between pancreatic NO and SS. The aim of this study was to determine whether L-arginine (L-Arg), the...

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Detalles Bibliográficos
Autores: Rodríguez Martín, Eulalia, Muñoz Acedo, Gema, Puebla Jiménez, Lilian|||0000-0002-0681-3235, Arilla Ferreiro, Eduardo
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/2175
Acceso en línea:http://hdl.handle.net/10017/2175
https://dx.doi.org/10.1016/S0167-4889(99)00032-4
Access Level:acceso abierto
Palabra clave:Nitric oxide
Somatostatin receptor
Adenylyl cyclase
Somatostatin-like immunoreactivity
Pancreatic acinar membrane
Rat
Bioquímica
Ciencia
Biochemistry
Science
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spelling Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreasRodríguez Martín, EulaliaMuñoz Acedo, GemaPuebla Jiménez, Lilian|||0000-0002-0681-3235Arilla Ferreiro, EduardoNitric oxideSomatostatin receptorAdenylyl cyclaseSomatostatin-like immunoreactivityPancreatic acinar membraneRatBioquímicaCienciaBiochemistryScienceNitric oxide (NO) and somatostatin (SS) are two important mediators of the exocrine and endocrine pancreas, exerting opposite effects on this organ. There is strong evidence suggesting an interaction between pancreatic NO and SS. The aim of this study was to determine whether L-arginine (L-Arg), the substrate for NO synthase (NOS), and Nω-nitro-L-arginine methyl ester (L-NAME), a NOS inhibitor, regulate pancreatic somatostatin-like immunoreactivity (SSLI) content and the SS mechanism of action in pancreatic acinar cell membranes. L-Arg (150 mg/kg, intraperitoneally (i.p.)), L-NAME (50 mg/kg, i.p.) or L-NAME plus L-Arg were injected twice daily at 8 h intervals for 8 days. L-Arg decreased pancreatic SSLI content as well as the number of SS receptors in pancreatic acinar cell membranes whereas L-NAME increased both parameters. The stable SS analogue SMS 201-995 induced a significantly lower inhibition of forskolin-stimulated adenylyl cyclase activity in pancreatic acinar cell membranes from L-Arg-treated rats whereas an increased inhibition was observed in pancreatic acinar membranes from L-NAME-treated rats. These results indicate that the NO system may contribute to the regulation of the pancreatic somatostatinergic system.Ministerio de Educación y CulturaElsevier19991999-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/2175https://dx.doi.org/10.1016/S0167-4889(99)00032-4reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATAopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/21752026-06-18T11:13:07Z
dc.title.none.fl_str_mv Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
title Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
spellingShingle Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
Rodríguez Martín, Eulalia
Nitric oxide
Somatostatin receptor
Adenylyl cyclase
Somatostatin-like immunoreactivity
Pancreatic acinar membrane
Rat
Bioquímica
Ciencia
Biochemistry
Science
title_short Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
title_full Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
title_fullStr Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
title_full_unstemmed Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
title_sort Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
dc.creator.none.fl_str_mv Rodríguez Martín, Eulalia
Muñoz Acedo, Gema
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author Rodríguez Martín, Eulalia
author_facet Rodríguez Martín, Eulalia
Muñoz Acedo, Gema
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author_role author
author2 Muñoz Acedo, Gema
Puebla Jiménez, Lilian|||0000-0002-0681-3235
Arilla Ferreiro, Eduardo
author2_role author
author
author
dc.subject.none.fl_str_mv Nitric oxide
Somatostatin receptor
Adenylyl cyclase
Somatostatin-like immunoreactivity
Pancreatic acinar membrane
Rat
Bioquímica
Ciencia
Biochemistry
Science
topic Nitric oxide
Somatostatin receptor
Adenylyl cyclase
Somatostatin-like immunoreactivity
Pancreatic acinar membrane
Rat
Bioquímica
Ciencia
Biochemistry
Science
description Nitric oxide (NO) and somatostatin (SS) are two important mediators of the exocrine and endocrine pancreas, exerting opposite effects on this organ. There is strong evidence suggesting an interaction between pancreatic NO and SS. The aim of this study was to determine whether L-arginine (L-Arg), the substrate for NO synthase (NOS), and Nω-nitro-L-arginine methyl ester (L-NAME), a NOS inhibitor, regulate pancreatic somatostatin-like immunoreactivity (SSLI) content and the SS mechanism of action in pancreatic acinar cell membranes. L-Arg (150 mg/kg, intraperitoneally (i.p.)), L-NAME (50 mg/kg, i.p.) or L-NAME plus L-Arg were injected twice daily at 8 h intervals for 8 days. L-Arg decreased pancreatic SSLI content as well as the number of SS receptors in pancreatic acinar cell membranes whereas L-NAME increased both parameters. The stable SS analogue SMS 201-995 induced a significantly lower inhibition of forskolin-stimulated adenylyl cyclase activity in pancreatic acinar cell membranes from L-Arg-treated rats whereas an increased inhibition was observed in pancreatic acinar membranes from L-NAME-treated rats. These results indicate that the NO system may contribute to the regulation of the pancreatic somatostatinergic system.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/2175
https://dx.doi.org/10.1016/S0167-4889(99)00032-4
url http://hdl.handle.net/10017/2175
https://dx.doi.org/10.1016/S0167-4889(99)00032-4
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia Not available PM95-0041 EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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