Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom

The aim of this study was to investigate the presence in the United Kingdom (UK) of Salmonella enterica serovar Typhimurium isolates carrying pUO-StVR2-like virulence-resistance hybrid plasmids that originated from pSLT. One hundred and fifty ampicillin-resistant isolates of S. Typhimurium, collecte...

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Authors: Fresno Herrero, Ana, Mendoza Fernández, M. Carmen, Threlfall, E. J., Rodicio, M. R.
Format: article
Publication Date:2009
Country:España
Institution:Universidad de Santiago de Compostela (USC)
Repository:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Language:English
OAI Identifier:oai:minerva.usc.gal:10347/45530
Online Access:https://hdl.handle.net/10347/45530
Access Level:Open access
Keyword:Salmonella enterica
Serotype Typhimurium
Multidrug resistance
Nitrofurantoin
Hybrid plasmid
pUO-StVR2
PFGE
24 Ciencias de la vida
2414 Microbiología
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spelling Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United KingdomFresno Herrero, AnaMendoza Fernández, M. CarmenThrelfall, E. J.Rodicio, M. R.Salmonella entericaSerotype TyphimuriumMultidrug resistanceNitrofurantoinHybrid plasmidpUO-StVR2PFGE24 Ciencias de la vida2414 MicrobiologíaThe aim of this study was to investigate the presence in the United Kingdom (UK) of Salmonella enterica serovar Typhimurium isolates carrying pUO-StVR2-like virulence-resistance hybrid plasmids that originated from pSLT. One hundred and fifty ampicillin-resistant isolates of S. Typhimurium, collected in different regions of the UK during 2006, were screened for the presence of bla OXA-1 carried by an InH-like integron (2000 bp/bla OXA-1-aadA1) characteristic of pUO-StVR2. Positive isolates were tested for the presence of a large plasmid that hybridised with probes specific for the bla OXA-1 and spvC genes, used as resistance and virulence markers of the hybrid plasmid, respectively. Eleven out of the 150 isolates fulfilled both criteria and were assigned to the S. Typhimurium pUO-StVR2 group. Nine were resistant to ampicillin, chloramphenicol, streptomycin/spectinomycin, sulfonamides and tetracycline, encoded by bla OXA-1, catA1, aadA1-like, sul1 and tet(B), respectively, and carried a pUO-StVR2-like plasmid of ca. 130 kb. Two contained hybrid plasmids of smaller size and lacked resistance(s) to chloramphenicol or chloramphenicol and tetracycline. The eleven isolates, which showed five and six closely related XbaI and BlnI profiles, respectively, were resistant to nitrofurantoin. In conclusion, multidrug-resistant S. Typhimurium isolates of the pUO-StVR2 group, which are endemic in Spain, were also detected in the UK, albeit with a low frequency (7.3%)SpringerUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular20092009-05-1220092009-05-12journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/45530reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/455302026-06-15T12:47:27Z
dc.title.none.fl_str_mv Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
title Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
spellingShingle Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
Fresno Herrero, Ana
Salmonella enterica
Serotype Typhimurium
Multidrug resistance
Nitrofurantoin
Hybrid plasmid
pUO-StVR2
PFGE
24 Ciencias de la vida
2414 Microbiología
title_short Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
title_full Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
title_fullStr Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
title_full_unstemmed Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
title_sort Detection of Salmonella enterica serotype Typhimurium with pUO-StVR2-like virulence-resistance hybrid plasmids in the United Kingdom
dc.creator.none.fl_str_mv Fresno Herrero, Ana
Mendoza Fernández, M. Carmen
Threlfall, E. J.
Rodicio, M. R.
author Fresno Herrero, Ana
author_facet Fresno Herrero, Ana
Mendoza Fernández, M. Carmen
Threlfall, E. J.
Rodicio, M. R.
author_role author
author2 Mendoza Fernández, M. Carmen
Threlfall, E. J.
Rodicio, M. R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
dc.subject.none.fl_str_mv Salmonella enterica
Serotype Typhimurium
Multidrug resistance
Nitrofurantoin
Hybrid plasmid
pUO-StVR2
PFGE
24 Ciencias de la vida
2414 Microbiología
topic Salmonella enterica
Serotype Typhimurium
Multidrug resistance
Nitrofurantoin
Hybrid plasmid
pUO-StVR2
PFGE
24 Ciencias de la vida
2414 Microbiología
description The aim of this study was to investigate the presence in the United Kingdom (UK) of Salmonella enterica serovar Typhimurium isolates carrying pUO-StVR2-like virulence-resistance hybrid plasmids that originated from pSLT. One hundred and fifty ampicillin-resistant isolates of S. Typhimurium, collected in different regions of the UK during 2006, were screened for the presence of bla OXA-1 carried by an InH-like integron (2000 bp/bla OXA-1-aadA1) characteristic of pUO-StVR2. Positive isolates were tested for the presence of a large plasmid that hybridised with probes specific for the bla OXA-1 and spvC genes, used as resistance and virulence markers of the hybrid plasmid, respectively. Eleven out of the 150 isolates fulfilled both criteria and were assigned to the S. Typhimurium pUO-StVR2 group. Nine were resistant to ampicillin, chloramphenicol, streptomycin/spectinomycin, sulfonamides and tetracycline, encoded by bla OXA-1, catA1, aadA1-like, sul1 and tet(B), respectively, and carried a pUO-StVR2-like plasmid of ca. 130 kb. Two contained hybrid plasmids of smaller size and lacked resistance(s) to chloramphenicol or chloramphenicol and tetracycline. The eleven isolates, which showed five and six closely related XbaI and BlnI profiles, respectively, were resistant to nitrofurantoin. In conclusion, multidrug-resistant S. Typhimurium isolates of the pUO-StVR2 group, which are endemic in Spain, were also detected in the UK, albeit with a low frequency (7.3%)
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-05-12
2009
2009-05-12
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/45530
url https://hdl.handle.net/10347/45530
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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