The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets

Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compa...

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Autores: Goršak, Tanja, Jarc Jovičić, Eva, Tratnjek, Larisa, Sepúlveda, Borja, Nogués, Josep, Kreft, Mateja Erdani, Petan, Toni, Kralj, Slavko, Makovec, Darko
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/356793
Acesso em linha:http://hdl.handle.net/10261/356793
Access Level:acceso abierto
Palavra-chave:Magneto-mechanical actuation
Hexaferrite nanoplatelets
Cell biavility
Cancer
Colloidal stability
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spelling The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplateletsGoršak, TanjaJarc Jovičić, EvaTratnjek, LarisaSepúlveda, BorjaNogués, JosepKreft, Mateja ErdaniPetan, ToniKralj, SlavkoMakovec, DarkoMagneto-mechanical actuationHexaferrite nanoplateletsCell biavilityCancerColloidal stabilityMagneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ∼50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1-2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations.The support of the Slovenian Research and Innovation Agency (ARIS) within the Project L2-3040, P2-0089, P1-0207 and P3-0108 is acknowledged. BS and JN acknowledge the support of the PID2019-106229RB-I00 grant by MCIN/AEI/10.13039/ and the 2021-SGR-00651 project by the Generalitat de Catalunya. ICN2 is funded by the CERCA programme/Generalitat de Catalunya. The ICN2 is supported by the CEX2021-001214-S grant funded by MCIN/AEI/10.13039/501100011033.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001214-S).Peer reviewedElsevierSlovenian Research AgencyAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Generalitat de CatalunyaMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/356793reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001214-Sinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106229RB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.jcis.2023.12.019https://doi.org/10.1016/j.jcis.2023.12.019Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3567932026-05-22T06:33:51Z
dc.title.none.fl_str_mv The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
title The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
spellingShingle The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
Goršak, Tanja
Magneto-mechanical actuation
Hexaferrite nanoplatelets
Cell biavility
Cancer
Colloidal stability
title_short The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
title_full The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
title_fullStr The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
title_full_unstemmed The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
title_sort The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
dc.creator.none.fl_str_mv Goršak, Tanja
Jarc Jovičić, Eva
Tratnjek, Larisa
Sepúlveda, Borja
Nogués, Josep
Kreft, Mateja Erdani
Petan, Toni
Kralj, Slavko
Makovec, Darko
author Goršak, Tanja
author_facet Goršak, Tanja
Jarc Jovičić, Eva
Tratnjek, Larisa
Sepúlveda, Borja
Nogués, Josep
Kreft, Mateja Erdani
Petan, Toni
Kralj, Slavko
Makovec, Darko
author_role author
author2 Jarc Jovičić, Eva
Tratnjek, Larisa
Sepúlveda, Borja
Nogués, Josep
Kreft, Mateja Erdani
Petan, Toni
Kralj, Slavko
Makovec, Darko
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Slovenian Research Agency
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Generalitat de Catalunya
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Magneto-mechanical actuation
Hexaferrite nanoplatelets
Cell biavility
Cancer
Colloidal stability
topic Magneto-mechanical actuation
Hexaferrite nanoplatelets
Cell biavility
Cancer
Colloidal stability
description Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ∼50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1-2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/356793
url http://hdl.handle.net/10261/356793
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001214-S
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106229RB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.jcis.2023.12.019
https://doi.org/10.1016/j.jcis.2023.12.019
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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