Autoantibody screening in Guillain-Barre syndrome
Background Guillain-Barre syndrome (GBS) is an acute inflammatory neuropathy with a heterogeneous presentation. Although some evidences support the role of autoantibodies in its pathogenesis, the target antigens remain unknown in a substantial proportion of GBS patients. The objective of this study...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p4234 |
| Acceso en línea: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4234 |
| Access Level: | acceso abierto |
| Palabra clave: | Guillain-Barre syndrome (GBS) Autoantibodies Anti-ganglioside Neurons Prognosis |
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Autoantibody screening in Guillain-Barre syndromeLleixa, CMartin-Aguilar, LPascual-Goni, EFranco, TCaballero, MDe Luna, NGallardo, ESuarez-Calvet, XMartinez-Martinez, LDiaz-Manera, JRojas-Garcia, RCortes-Vicente, ETuron, JCasasnovas, CHomedes, CGutierrez-Gutierrez, GJimeno-Montero, MCBerciano, JSedano-Tous, MJGarcia-Sobrino, TPardo-Fernandez, JMarquez-Infante, CRojas-Marcos, IJerico-Pascual, IMartinez-Hernandez, Ede la Tassa, GMDominguez-Gonzalez, CJuarez, CIlla, IQuerol, LGuillain-Barre syndrome (GBS)AutoantibodiesAnti-gangliosideNeuronsPrognosisBackground Guillain-Barre syndrome (GBS) is an acute inflammatory neuropathy with a heterogeneous presentation. Although some evidences support the role of autoantibodies in its pathogenesis, the target antigens remain unknown in a substantial proportion of GBS patients. The objective of this study is to screen for autoantibodies targeting peripheral nerve components in Guillain-Barre syndrome. Methods Autoantibody screening was performed in serum samples from all GBS patients included in the International GBS Outcome study by 11 different Spanish centres. The screening included testing for anti-ganglioside antibodies, anti-nodo/paranodal antibodies, immunocytochemistry on neuroblastoma-derived human motor neurons and murine dorsal root ganglia (DRG) neurons, and immunohistochemistry on monkey peripheral nerve sections. We analysed the staining patterns of patients and controls. The prognostic value of anti-ganglioside antibodies was also analysed. Results None of the GBS patients (n = 100) reacted against the nodo/paranodal proteins tested, and 61 (61%) were positive for, at least, one anti-ganglioside antibody. GBS sera reacted strongly against DRG neurons more frequently than controls both with IgG (6% vs 0%; p = 0.03) and IgM (11% vs 2.2%; p = 0.02) immunodetection. No differences were observed in the proportion of patients reacting against neuroblastoma-derived human motor neurons. Reactivity against monkey nerve tissue was frequently detected both in patients and controls, but specific patterns were only detected in GBS patients: IgG from 13 (13%) patients reacted strongly against Schwann cells. Finally, we confirmed that IgG anti-GM1 antibodies are associated with poorer outcomes independently of other known prognostic factors. Conclusion Our study confirms that (1) GBS patients display a heterogeneous repertoire of autoantibodies targeting nerve cells and structures; (2) gangliosides are the most frequent antigens in GBS patients and have a prognostic value; (3) further antigen-discovery experiments may elucidate other potential antigens in GBS.BMC2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4234Journal of NeuroinflammationISSN: 17422094reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p42342026-06-14T12:41:47Z |
| dc.title.none.fl_str_mv |
Autoantibody screening in Guillain-Barre syndrome |
| title |
Autoantibody screening in Guillain-Barre syndrome |
| spellingShingle |
Autoantibody screening in Guillain-Barre syndrome Lleixa, C Guillain-Barre syndrome (GBS) Autoantibodies Anti-ganglioside Neurons Prognosis |
| title_short |
Autoantibody screening in Guillain-Barre syndrome |
| title_full |
Autoantibody screening in Guillain-Barre syndrome |
| title_fullStr |
Autoantibody screening in Guillain-Barre syndrome |
| title_full_unstemmed |
Autoantibody screening in Guillain-Barre syndrome |
| title_sort |
Autoantibody screening in Guillain-Barre syndrome |
| dc.creator.none.fl_str_mv |
Lleixa, C Martin-Aguilar, L Pascual-Goni, E Franco, T Caballero, M De Luna, N Gallardo, E Suarez-Calvet, X Martinez-Martinez, L Diaz-Manera, J Rojas-Garcia, R Cortes-Vicente, E Turon, J Casasnovas, C Homedes, C Gutierrez-Gutierrez, G Jimeno-Montero, MC Berciano, J Sedano-Tous, MJ Garcia-Sobrino, T Pardo-Fernandez, J Marquez-Infante, C Rojas-Marcos, I Jerico-Pascual, I Martinez-Hernandez, E de la Tassa, GM Dominguez-Gonzalez, C Juarez, C Illa, I Querol, L |
| author |
Lleixa, C |
| author_facet |
Lleixa, C Martin-Aguilar, L Pascual-Goni, E Franco, T Caballero, M De Luna, N Gallardo, E Suarez-Calvet, X Martinez-Martinez, L Diaz-Manera, J Rojas-Garcia, R Cortes-Vicente, E Turon, J Casasnovas, C Homedes, C Gutierrez-Gutierrez, G Jimeno-Montero, MC Berciano, J Sedano-Tous, MJ Garcia-Sobrino, T Pardo-Fernandez, J Marquez-Infante, C Rojas-Marcos, I Jerico-Pascual, I Martinez-Hernandez, E de la Tassa, GM Dominguez-Gonzalez, C Juarez, C Illa, I Querol, L |
| author_role |
author |
| author2 |
Martin-Aguilar, L Pascual-Goni, E Franco, T Caballero, M De Luna, N Gallardo, E Suarez-Calvet, X Martinez-Martinez, L Diaz-Manera, J Rojas-Garcia, R Cortes-Vicente, E Turon, J Casasnovas, C Homedes, C Gutierrez-Gutierrez, G Jimeno-Montero, MC Berciano, J Sedano-Tous, MJ Garcia-Sobrino, T Pardo-Fernandez, J Marquez-Infante, C Rojas-Marcos, I Jerico-Pascual, I Martinez-Hernandez, E de la Tassa, GM Dominguez-Gonzalez, C Juarez, C Illa, I Querol, L |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Guillain-Barre syndrome (GBS) Autoantibodies Anti-ganglioside Neurons Prognosis |
| topic |
Guillain-Barre syndrome (GBS) Autoantibodies Anti-ganglioside Neurons Prognosis |
| description |
Background Guillain-Barre syndrome (GBS) is an acute inflammatory neuropathy with a heterogeneous presentation. Although some evidences support the role of autoantibodies in its pathogenesis, the target antigens remain unknown in a substantial proportion of GBS patients. The objective of this study is to screen for autoantibodies targeting peripheral nerve components in Guillain-Barre syndrome. Methods Autoantibody screening was performed in serum samples from all GBS patients included in the International GBS Outcome study by 11 different Spanish centres. The screening included testing for anti-ganglioside antibodies, anti-nodo/paranodal antibodies, immunocytochemistry on neuroblastoma-derived human motor neurons and murine dorsal root ganglia (DRG) neurons, and immunohistochemistry on monkey peripheral nerve sections. We analysed the staining patterns of patients and controls. The prognostic value of anti-ganglioside antibodies was also analysed. Results None of the GBS patients (n = 100) reacted against the nodo/paranodal proteins tested, and 61 (61%) were positive for, at least, one anti-ganglioside antibody. GBS sera reacted strongly against DRG neurons more frequently than controls both with IgG (6% vs 0%; p = 0.03) and IgM (11% vs 2.2%; p = 0.02) immunodetection. No differences were observed in the proportion of patients reacting against neuroblastoma-derived human motor neurons. Reactivity against monkey nerve tissue was frequently detected both in patients and controls, but specific patterns were only detected in GBS patients: IgG from 13 (13%) patients reacted strongly against Schwann cells. Finally, we confirmed that IgG anti-GM1 antibodies are associated with poorer outcomes independently of other known prognostic factors. Conclusion Our study confirms that (1) GBS patients display a heterogeneous repertoire of autoantibodies targeting nerve cells and structures; (2) gangliosides are the most frequent antigens in GBS patients and have a prognostic value; (3) further antigen-discovery experiments may elucidate other potential antigens in GBS. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4234 |
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https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4234 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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BMC |
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BMC |
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Journal of Neuroinflammation ISSN: 17422094 reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
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Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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