Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients

Multiple sclerosis (MS) is an autoimmune, inflammatory, and neurodegenerative disease of the central nervous system for which there is no cure, making it necessary to search for new treatments. The endocannabinoid system (ECS) plays a very important neuromodulatory role in the CNS. In recent years,...

Descripción completa

Detalles Bibliográficos
Autores: Menéndez-Pérez, Carlota, Rivas‐Santisteban, Rafael, del Valle, Eva, Tolivia, Jorge, Navarro, Ana, Franco Fernández, Rafael, Martínez-Pinilla, Eva
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/218496
Acceso en línea:https://hdl.handle.net/2445/218496
Access Level:acceso abierto
Palabra clave:Cànnabis
Esclerosi múltiple
Escorça frontal
Cannabis
Multiple sclerosis
Prefrontal cortex
id ES_232ff3b4e36cb63dd6f7a61d330c986d
oai_identifier_str oai:diposit.ub.edu:2445/218496
network_acronym_str ES
network_name_str España
repository_id_str
spelling Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis PatientsMenéndez-Pérez, CarlotaRivas‐Santisteban, Rafaeldel Valle, EvaTolivia, JorgeNavarro, AnaFranco Fernández, RafaelMartínez-Pinilla, EvaCànnabisEsclerosi múltipleEscorça frontalCannabisMultiple sclerosisPrefrontal cortexMultiple sclerosis (MS) is an autoimmune, inflammatory, and neurodegenerative disease of the central nervous system for which there is no cure, making it necessary to search for new treatments. The endocannabinoid system (ECS) plays a very important neuromodulatory role in the CNS. In recent years, the formation of heteromers containing cannabinoid receptors and their up/downregulation in some neurodegenerative diseases have been demonstrated. Despite the beneficial effects shown by some phytocannabinoids in MS, the role of the ECS in its pathophysiology is unknown. The main objective of this work was to identify heteromers of cell surface proteins receptive to cannabinoids, namely GPR55, CB1 and CB2 receptors, in brain samples from control subjects and MS patients, as well as determining their cellular localization, using In Situ Proximity Ligation Assays and immunohistochemical techniques. For the first time, CB1R-GPR55 and CB2R-GPR55 heteromers are identified in the prefrontal cortex of the human brain, more in the grey than in the white matter. Remarkably, the number of CB1R-GPR55 and CB2R-GPR55 complexes was found to be increased in MS patient samples. The results obtained open a promising avenue of research on the use of these receptor complexes as potential therapeutic targets for the disease.MDPI2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/218496Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms25084176International Journal of Molecular Sciences, 2024, vol. 25, num.8, p. 1-14https://doi.org/10.3390/ijms25084176cc-by (c) Menéndez-Pérez, C et al., 2024http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2184962026-05-27T06:46:51Z
dc.title.none.fl_str_mv Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
title Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
spellingShingle Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
Menéndez-Pérez, Carlota
Cànnabis
Esclerosi múltiple
Escorça frontal
Cannabis
Multiple sclerosis
Prefrontal cortex
title_short Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
title_full Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
title_fullStr Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
title_full_unstemmed Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
title_sort Heteromers Formed by GPR55 and Either Cannabinoid CB<sub>1</sub> or CB<sub>2</sub> Receptors Are Upregulated in the Prefrontal Cortex of Multiple Sclerosis Patients
dc.creator.none.fl_str_mv Menéndez-Pérez, Carlota
Rivas‐Santisteban, Rafael
del Valle, Eva
Tolivia, Jorge
Navarro, Ana
Franco Fernández, Rafael
Martínez-Pinilla, Eva
author Menéndez-Pérez, Carlota
author_facet Menéndez-Pérez, Carlota
Rivas‐Santisteban, Rafael
del Valle, Eva
Tolivia, Jorge
Navarro, Ana
Franco Fernández, Rafael
Martínez-Pinilla, Eva
author_role author
author2 Rivas‐Santisteban, Rafael
del Valle, Eva
Tolivia, Jorge
Navarro, Ana
Franco Fernández, Rafael
Martínez-Pinilla, Eva
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cànnabis
Esclerosi múltiple
Escorça frontal
Cannabis
Multiple sclerosis
Prefrontal cortex
topic Cànnabis
Esclerosi múltiple
Escorça frontal
Cannabis
Multiple sclerosis
Prefrontal cortex
description Multiple sclerosis (MS) is an autoimmune, inflammatory, and neurodegenerative disease of the central nervous system for which there is no cure, making it necessary to search for new treatments. The endocannabinoid system (ECS) plays a very important neuromodulatory role in the CNS. In recent years, the formation of heteromers containing cannabinoid receptors and their up/downregulation in some neurodegenerative diseases have been demonstrated. Despite the beneficial effects shown by some phytocannabinoids in MS, the role of the ECS in its pathophysiology is unknown. The main objective of this work was to identify heteromers of cell surface proteins receptive to cannabinoids, namely GPR55, CB1 and CB2 receptors, in brain samples from control subjects and MS patients, as well as determining their cellular localization, using In Situ Proximity Ligation Assays and immunohistochemical techniques. For the first time, CB1R-GPR55 and CB2R-GPR55 heteromers are identified in the prefrontal cortex of the human brain, more in the grey than in the white matter. Remarkably, the number of CB1R-GPR55 and CB2R-GPR55 complexes was found to be increased in MS patient samples. The results obtained open a promising avenue of research on the use of these receptor complexes as potential therapeutic targets for the disease.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218496
url https://hdl.handle.net/2445/218496
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms25084176
International Journal of Molecular Sciences, 2024, vol. 25, num.8, p. 1-14
https://doi.org/10.3390/ijms25084176
dc.rights.none.fl_str_mv cc-by (c) Menéndez-Pérez, C et al., 2024
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Menéndez-Pérez, C et al., 2024
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404626034360320
score 15.81155

Similares en LA Referencia