A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress

Cells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-st...

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Detalles Bibliográficos
Autores: Viéitez, Cristina, Martínez Cebrián, Gerard, Solé Serra, Carme, Böttcher, René, Potel, Clement M., Savitski, Mikhail M., Onnebo, Sara, Fabregat, Marc, Shilatifard, Ali, Posas, Francesc, Nadal Clanchet, Eulàlia De
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/220510
Acceso en línea:https://hdl.handle.net/2445/220510
Access Level:acceso abierto
Palabra clave:Cèl·lules
Adaptació (Biologia)
Estrès (Fisiologia)
Histones
Cells
Adaptation (Biology)
Stress (Physiology)
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spelling A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stressViéitez, CristinaMartínez Cebrián, GerardSolé Serra, CarmeBöttcher, RenéPotel, Clement M.Savitski, Mikhail M.Onnebo, SaraFabregat, MarcShilatifard, AliPosas, FrancescNadal Clanchet, Eulàlia DeCèl·lulesAdaptació (Biologia)Estrès (Fisiologia)HistonesCellsAdaptation (Biology)Stress (Physiology)HistonesCells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-state growth conditions. Comparatively less is known about the role of histone PTMs in the cellular adaptive response to stress. Here, we performed high-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress. Of note, we observed that the histone residues needed depend on the type of gene and/or stress, thereby suggesting a 'personalized', rather than general, subset of histone requirements for each chromatin context. In addition, we identified a number of new residues that unexpectedly serve to regulate transcription. As a proof of concept, we characterized the function of the histone residues H4-S47 and H4-T30 in response to osmotic and heat stress, respectively. Our results uncover novel roles for the kinases Cla4 and Ste20, yeast homologs of the mammalian PAK2 family, and the Ste11 MAPK as regulators of H4-S47 and H4-T30, respectively. This study provides new insights into the role of histone residues in transcriptional regulation under stress conditions.Oxford University Press2025202520202025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion21 p.application/pdfhttps://hdl.handle.net/2445/220510Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1093/nar/gkaa081Nucleic Acids Research, 2020, vol. 48, num. 7, p. 3455-3475https://doi.org/10.1093/nar/gkaa081cc-by (c) Viéitez, Cristina et al., 2020http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2205102026-05-29T05:05:01Z
dc.title.none.fl_str_mv A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
title A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
spellingShingle A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
Viéitez, Cristina
Cèl·lules
Adaptació (Biologia)
Estrès (Fisiologia)
Histones
Cells
Adaptation (Biology)
Stress (Physiology)
Histones
title_short A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
title_full A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
title_fullStr A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
title_full_unstemmed A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
title_sort A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
dc.creator.none.fl_str_mv Viéitez, Cristina
Martínez Cebrián, Gerard
Solé Serra, Carme
Böttcher, René
Potel, Clement M.
Savitski, Mikhail M.
Onnebo, Sara
Fabregat, Marc
Shilatifard, Ali
Posas, Francesc
Nadal Clanchet, Eulàlia De
author Viéitez, Cristina
author_facet Viéitez, Cristina
Martínez Cebrián, Gerard
Solé Serra, Carme
Böttcher, René
Potel, Clement M.
Savitski, Mikhail M.
Onnebo, Sara
Fabregat, Marc
Shilatifard, Ali
Posas, Francesc
Nadal Clanchet, Eulàlia De
author_role author
author2 Martínez Cebrián, Gerard
Solé Serra, Carme
Böttcher, René
Potel, Clement M.
Savitski, Mikhail M.
Onnebo, Sara
Fabregat, Marc
Shilatifard, Ali
Posas, Francesc
Nadal Clanchet, Eulàlia De
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules
Adaptació (Biologia)
Estrès (Fisiologia)
Histones
Cells
Adaptation (Biology)
Stress (Physiology)
Histones
topic Cèl·lules
Adaptació (Biologia)
Estrès (Fisiologia)
Histones
Cells
Adaptation (Biology)
Stress (Physiology)
Histones
description Cells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-state growth conditions. Comparatively less is known about the role of histone PTMs in the cellular adaptive response to stress. Here, we performed high-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress. Of note, we observed that the histone residues needed depend on the type of gene and/or stress, thereby suggesting a 'personalized', rather than general, subset of histone requirements for each chromatin context. In addition, we identified a number of new residues that unexpectedly serve to regulate transcription. As a proof of concept, we characterized the function of the histone residues H4-S47 and H4-T30 in response to osmotic and heat stress, respectively. Our results uncover novel roles for the kinases Cla4 and Ste20, yeast homologs of the mammalian PAK2 family, and the Ste11 MAPK as regulators of H4-S47 and H4-T30, respectively. This study provides new insights into the role of histone residues in transcriptional regulation under stress conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/220510
url https://hdl.handle.net/2445/220510
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1093/nar/gkaa081
Nucleic Acids Research, 2020, vol. 48, num. 7, p. 3455-3475
https://doi.org/10.1093/nar/gkaa081
dc.rights.none.fl_str_mv cc-by (c) Viéitez, Cristina et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Viéitez, Cristina et al., 2020
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21 p.
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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