Multi-Component 3D bioprinted platform with sacrificial matrix and collagen-based bioinks for skeletal muscle tissue engineering
The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE)...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:dnet:idus________::bc6de2e8caabf79946bc10d4cf57b525 |
| Acceso en línea: | https://hdl.handle.net/11441/186708 https://doi.org/10.3390/polym18101223 |
| Access Level: | acceso abierto |
| Palabra clave: | 3D bioprinting skeletal muscle tissue engineering rheology collagen-based bioinks extracellular matrix scaffolds biofabrication |
| Sumario: | The development of biomimetic and mechanically functional constructs remains a major challenge in skeletal muscle tissue engineering. In this study, we present a multi-component 3D bioprinted platform integrating a polycaprolactone (PCL) support for mechanical stimulation, a sacrificial gelatin (GE) matrix for controlled bioink deposition, and collagen-based bioinks laden with Rattus norvegicus L6 skeletal muscle cells. The influence of PCL architecture, GE concentration (0.75, 1.5 and 3 wt%), and bioink composition—collagen (C), collagen–Matrigel (CM), and extracellular matrix-based (ECM)—was systematically evaluated. Rheological characterization demonstrated that all bioinks exhibited shear-thinning behavior and suitable viscoelastic properties for extrusion-based bioprinting, with sufficient mechanical stability to withstand dynamic bioreactor conditions. Microstructural analysis revealed highly interconnected porous networks, particularly in ECM-based scaffolds. While no statistically significant differences were observed, the ECM-based bioinks showed the highest cell viability and improved structural organization. Overall, this work demonstrates a versatile bioprinting strategy that combines mechanical support and biomimetic environments, highlighting the potential of ECM-based bioinks for the fabrication of functional skeletal muscle constructs. |
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