Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA

The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from p...

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Bibliographic Details
Authors: Albesa Jové, David, Svetlıkova, Zuzana, Tersa Peñacoba, Montserrat, Sancho Vaello, Enea, Carreras González, Ana, Bonnet, Pascal, Arrasate Bermeosolo, Pedro, Eguskiza, Ander, Angala, Shiva K., Cifuente, Javier O., Kordulakova, Jana, Jackson, Mary, Mikusova, Katarına, Guerín, Marcelo Eduardo
Format: article
Publication Date:2016
Country:España
Institution:Universidad del País Vasco
Repository:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/24827
Online Access:http://hdl.handle.net/10810/24827
Access Level:Open access
Keyword:squash glycerol-3-phosphate(1)-acyltransferase
bacillus-calmette-guerin
fatty-acid biosynthesis
phosphatidylinositol mannosides
mycobacterium phlei
SN-glycerol-3-phosphate acyltransferase
escherichia-coli
acylation state
molecular-basis
mannosyltransferase PimA
Description
Summary:The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an alpha/beta architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design.

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