Puesta a punto de un sistema de nanopartículas de sílice mesoporosa para edición génica basado en la tecnología CRISPR/Cas9
The aim of this project is to optimize a novel nanodispositive based on silica mesoporous nanoparticles (MSNs) for genome editing in human cell lines, using CRISPR/Cas9 technology. It focuses on the synthesis and characterization of the mesoporous silica nanparticles capped with the CRISPR/Cas9 plas...
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| Tipo de documento: | dissertação |
| Data de publicação: | 2017 |
| País: | España |
| Recursos: | Universitat Politècnica de València (UPV) |
| Repositório: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | espanhol |
| OAI Identifier: | oai:riunet.upv.es:10251/89997 |
| Acesso em linha: | https://riunet.upv.es/handle/10251/89997 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Genome editing Silica mesoporous nanoparticles Edición génica Nanopartículas de sílice mesoporosa CRISPR/Cas9 BIOQUIMICA Y BIOLOGIA MOLECULAR Máster Universitario en Biotecnología Biomédica-Màster Universitari en Biotecnologia Biomèdica |
| Resumo: | The aim of this project is to optimize a novel nanodispositive based on silica mesoporous nanoparticles (MSNs) for genome editing in human cell lines, using CRISPR/Cas9 technology. It focuses on the synthesis and characterization of the mesoporous silica nanparticles capped with the CRISPR/Cas9 plasmid. The work is focused on the optimization of the CRISPR/Cas9 plasmid construction, which targets green fluorescent protein (GFP), as well as on the optimization of the GFP editing conditions in human cell lines using the MSNs as a carrier for plasmid delivery. |
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