High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells

Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects...

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Autores: Searle, Andrea, Gómez Rosso, Leonardo, Meroño, Tomás, Salomon, Carlos, Durán-Sandoval, Daniel, Giunta, Gustavo, Grant, Carlos, Calvo, Carlos, Lamperti, Liliana, Brites, Fernando, Aguayo, Claudio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/193701
Acceso en línea:https://hdl.handle.net/2445/193701
Access Level:acceso abierto
Palabra clave:Lipoproteïnes
Colesterol
Malalties cardiovasculars
Lipoproteins
Cholesterol
Cardiovascular diseases
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spelling High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cellsSearle, AndreaGómez Rosso, LeonardoMeroño, TomásSalomon, CarlosDurán-Sandoval, DanielGiunta, GustavoGrant, CarlosCalvo, CarlosLamperti, LilianaBrites, FernandoAguayo, ClaudioLipoproteïnesColesterolMalalties cardiovascularsLipoproteinsCholesterolCardiovascular diseasesObjective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDL-cholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA(2) activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA(2) activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA(2) carrier, and not to LDL composition or intrinsic Lp-PLA(2) activity.Elsevier B.V.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/193701Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.clinbiochem.2010.10.004Clinical Biochemistry, 2011, vol. 44, num. 2, p. 171-177https://doi.org/10.1016/j.clinbiochem.2010.10.004(c) Elsevier B.V., 2011info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1937012026-05-27T06:46:51Z
dc.title.none.fl_str_mv High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
spellingShingle High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
Searle, Andrea
Lipoproteïnes
Colesterol
Malalties cardiovasculars
Lipoproteins
Cholesterol
Cardiovascular diseases
title_short High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_full High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_fullStr High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_full_unstemmed High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
title_sort High LDL levels are associated with increased lipoprotein-associated phospholipase A(2) activity on nitric oxide synthesis and reactive oxygen species formation in human endothelial cells
dc.creator.none.fl_str_mv Searle, Andrea
Gómez Rosso, Leonardo
Meroño, Tomás
Salomon, Carlos
Durán-Sandoval, Daniel
Giunta, Gustavo
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando
Aguayo, Claudio
author Searle, Andrea
author_facet Searle, Andrea
Gómez Rosso, Leonardo
Meroño, Tomás
Salomon, Carlos
Durán-Sandoval, Daniel
Giunta, Gustavo
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando
Aguayo, Claudio
author_role author
author2 Gómez Rosso, Leonardo
Meroño, Tomás
Salomon, Carlos
Durán-Sandoval, Daniel
Giunta, Gustavo
Grant, Carlos
Calvo, Carlos
Lamperti, Liliana
Brites, Fernando
Aguayo, Claudio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lipoproteïnes
Colesterol
Malalties cardiovasculars
Lipoproteins
Cholesterol
Cardiovascular diseases
topic Lipoproteïnes
Colesterol
Malalties cardiovasculars
Lipoproteins
Cholesterol
Cardiovascular diseases
description Objective: To evaluate in vitro the effects of serum and LDL fractions isolated from hypercholesterolemic patients on nitric oxide (NO) synthesis and reactive oxygen species (ROS) production by human umbilical vein endothelial cells (HUVECs). Design and methods: Serum and LDL isolated from subjects with high (n=18) and normal (n=21) LDL-cholesterol levels were analyzed on NO synthesis and ROS production in vitro models of HUVECs. LDL was furthers characterized in their chemical composition and activities of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), cholesteryl ester transfer protein (CETP) and paraoxonase. Results: NO bioavailability was significantly lower and ROS production higher in HUVECs incubated with serum samples from patients with high LDL-cholesterol levels in comparison to control subjects. Moreover, hypercholesterolemic patients presented higher CETP and Lp-PLA(2) activities than control subjects. LDL fractions isolated from patients and controls were not different in their chemical composition, Lp-PLA(2) activity, and their capacity to reduce NO synthesis and increase ROS production. Conclusion: Alterations of serum from hypercholesterolemic patients could be due to the increment in LDL concentration, main Lp-PLA(2) carrier, and not to LDL composition or intrinsic Lp-PLA(2) activity.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/193701
url https://hdl.handle.net/2445/193701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.clinbiochem.2010.10.004
Clinical Biochemistry, 2011, vol. 44, num. 2, p. 171-177
https://doi.org/10.1016/j.clinbiochem.2010.10.004
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2011
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2011
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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