Emergence of Neisseria meningitidis ST-3587 harbouring bla ROB-1 and exhibiting dual resistance to penicillin and ciprofloxacin, Spain, 2024

Background: Dual penicillin-and ciprofloxacinresistant Neisseria meningitidis causing invasive meningococcal disease (IMD) have recently emerged in association with sequence type (ST) 3587, harbouring ROB-1 beta-lactamase (bla ROB-1) and a mutated DNA gyrase (gyrA). These strains pose a threat to cu...

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Bibliographic Details
Authors: Roca Grande, Josep, Moreno Mingorance, Albert, Bellés Bellés, Alba, Burgos, Joaquín, Càmara Mas, Jordi, Hoyos Mallecot, Yannick, López Alcaide, Lucía, López Madueño, Joan, Lung, Mayli, Martín Nalda, Andrea, Mir Cros, Alba, Muñoz Almagro, Carmen, Pérez Argüello, Amaresh, Puigsech Boixeda, Guillem, Quesada, M. Dolores, Sarvisé, Carolina, Soler García, Aleix, Soler Palacín, Pere, Trejo Zahínos, Jesús, Trujillo, Gloria, Viñado, Belén, Larrosa, M Nieves, González López, Juan José
Format: article
Status:Published version
Publication Date:2026
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:dnet:recercat____::d6747874861b6da3035f8c386bbf80ef
Online Access:https://hdl.handle.net/2445/229309
Access Level:Open access
Keyword:Penicil·lina
Resistència als medicaments
Infeccions oportunistes
Salut pública
Penicillin
Drug resistance
Opportunistic infections
Public health
Description
Summary:Background: Dual penicillin-and ciprofloxacinresistant Neisseria meningitidis causing invasive meningococcal disease (IMD) have recently emerged in association with sequence type (ST) 3587, harbouring ROB-1 beta-lactamase (bla ROB-1) and a mutated DNA gyrase (gyrA). These strains pose a threat to current antimicrobial treatment and prophylaxis. Aim: We aimed to characterise the first dual-resistant N. meningitidis ST-3587 isolates harbouring bla ROB-1 and a mutated gyrA identified in Spain. Methods: Three N. meningitidis isolates encoding bla ROB-1 were identified in 2024. They were characterised by whole genome sequencing to determine capsular genogroups, ST and genetic antimicrobial resistance markers. Dated phylogenetic analysis was performed alongside global ST-3587 strains. Results: The three bla ROB-1-encoding isolates belonged to ST-3587, genogroup Y, harboured a T91I mutation in gyrA and showed resistance to penicillin and ciprofloxacin. These isolates were obtained from urethral, oropharyngeal and blood samples, each from a different patient. According to the dated phylogenetic analysis of ST-3587 and the presence of bla ROB-1, two clades were defined: clade I and clade II. Within clade II, subclade II.I was identified, comprising isolates which, in addition to bla ROB-1, carried the T91I mutation in gyrA. This subclade included the three Spanish isolates, which exhibited close genetic relatedness.Conclusion: This study documents the emergence of N. meningitidis ST-3587 with dual resistance in Europe, including a documented urogenital infection by this lineage. Continued surveillance of antimicrobial resistance in N. meningitidis, including non-invasive cases, is crucial for timely public health responses and effective IMD prevention strategies.

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