Allergen sensitization stratifies IL-31 production by memory T cells in atopic dermatitis patients

Background: The role of allergen sensitization in IL-31 production by T cells and specifically in the clinical context of atopic dermatitis (AD) has not been characterized. Methods: The response to house dust mite (HDM) in purified memory T cells cocultured with epidermal cells from AD patients (n=5...

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Detalles Bibliográficos
Autores: Sans-de San Nicolás, Lídia, Figueras-Nart, Ignasi, García-Jiménez, Irene, Bonfill-Ortí, Montserrat, Guilabert, Antonio, Curto Barredo, Laia, Bertolín Colilla, Marta, Ferran Farrés, Marta, Serra-Baldrich, Esther, Pujol Vallverdú, Ramón M., Santamaria-Babí, Luis F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/59322
Acceso en línea:http://hdl.handle.net/10230/59322
http://dx.doi.org/10.3389/fimmu.2023.1124018
Access Level:acceso abierto
Palabra clave:CCL27
CLA+ memory T cells
IL-31
IgE
Atopic dermatitis
House dust mite
Periostin
Pruritus
Descripción
Sumario:Background: The role of allergen sensitization in IL-31 production by T cells and specifically in the clinical context of atopic dermatitis (AD) has not been characterized. Methods: The response to house dust mite (HDM) in purified memory T cells cocultured with epidermal cells from AD patients (n=58) and control subjects (n=11) was evaluated. AD-associated cytokines from culture supernatants, plasma proteins and mRNA expression from cutaneous lesions were assessed and related with the clinical features of the patients. Results: HDM-induced IL-31 production by memory T cells defined two subsets of AD patients according to the presence or absence of IL-31 response. Patients in the IL-31 producing group showed a more inflammatory profile, and increased HDM-specific (sp) and total IgE levels compared to the IL-31 non-producing group. A correlation between IL-31 production and patient's pruritus intensity, plasma CCL27 and periostin was detected. When the same patients were analyzed based on sp IgE and total IgE levels, an increased IL-31 in vitro response, as well as type 2 markers in plasma and cutaneous lesions, was found in patients with sp IgE levels > 100 kUA/L and total IgE levels > 1000 kU/L. The IL-31 response by memory T cells was restricted to the cutaneous lymphocyte-associated antigen (CLA)+ T-cell subset. Conclusion: IgE sensitization to HDM allows stratifying IL-31 production by memory T cells in AD patients and relating it to particular clinical phenotypes of the disease.

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