The DNA methylation landscape of the root-knot nematode-induced pseudo-organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism

Root-knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large-scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation in Arabidopsis galls induced by Meloidogyne jav...

Descripción completa

Detalles Bibliográficos
Autores: Silva, Ana Cláudia, Ruiz Ferrer, Virginia, Müller, Sebastian Y., Pellegrin, Clement, Abril Urías, Patricia, Martínez Gómez, Ángela, Gómez Rojas, Almudena, Berenguer, Eduardo, Sánchez Testillano, Pilar, Andrés, Maria Fe, Fenoll Comes, María Carmen, Eves-van den Akker, Sebastian, Escobar Lucas, Carolina
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/36578
Acceso en línea:https://hdl.handle.net/10578/36578
Access Level:acceso abierto
Palabra clave:Arabidopsis
DNA methylation/ epigenetics signatures,
Galls
Giant cells
Meloidogyne javanica
Descripción
Sumario:Root-knot nematodes (RKNs) induce giant cells (GCs) within galls which are characterized by large-scale gene repression at early stages. However, the epigenetic mechanism(s) underlying gene silencing is (are) still poorly characterized. DNA methylation in Arabidopsis galls induced by Meloidogyne javanica was studied at crucial infection stages (3 d post-infection (dpi) and 14 dpi) using enzymatic, cytological, and sequencing approaches. DNA methyltransferase mutants (met1, cmt2, cmt3, cmt2/3, drm1/ 2, ddc) and a DNA demethylase mutant (ros1), were analyzed for RKN resistance/tolerance, and galls were characterized by confocal microscopy and RNA-seq.

Similares en LA Referencia