Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis

Arv1p is involved in the regulation of cellular lipid homeostasis in the yeast Saccharomyces cerevisiae. Here, we report the characterization of the two Arabidopsis thaliana ARV genes and the encoded proteins, AtArv1p and AtArv2p. The functional identity of AtArv1p and AtArv2p was demonstrated by co...

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Detalles Bibliográficos
Autores: Forés del Ruste, Oriol, Arró i Plans, Montserrat, Pahissa, Albert, Ferrero, Sergi, Germann, Melody, Stukey, Joseph, McDonough, Virgina, Nickels Jr., Joseph T., Campos Martínez, Narciso, Ferrer i Prats, Albert
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2006
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/225881
Acceso en línea:https://hdl.handle.net/2445/225881
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
Biologia molecular vegetal
Plant molecular biology
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spelling Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasisForés del Ruste, OriolArró i Plans, MontserratPahissa, AlbertFerrero, SergiGermann, MelodyStukey, JosephMcDonough, VirginaNickels Jr., Joseph T.Campos Martínez, NarcisoFerrer i Prats, AlbertArabidopsis thalianaBiologia molecular vegetalArabidopsis thalianaPlant molecular biologyArv1p is involved in the regulation of cellular lipid homeostasis in the yeast Saccharomyces cerevisiae. Here, we report the characterization of the two Arabidopsis thaliana ARV genes and the encoded proteins, AtArv1p and AtArv2p. The functional identity of AtArv1p and AtArv2p was demonstrated by complementation of the thermosensitive phenotype of the arv1Δ yeast mutant strain YJN1756. Both A. thaliana proteins contain the bipartite Arv1 homology domain (AHD), which consists of an NH2-terminal cysteine-rich subdomain with a putative zinc-binding motif followed by a C-terminal subdomain of 33 amino acids. Removal of the cysteine-rich subdomain has no effect on Arvp activity, whereas the presence of the C-terminal subdomain of the AHD is critical for Arvp function. Localization experiments of AtArv1p and AtArv2p tagged with green fluorescent protein (GFP) and expressed in onion epidermal cells demonstrated that both proteins are exclusively targeted to the endoplasmic reticulum. Analysis of β-glucuronidase (GUS) activity in transgenic A. thaliana plants carrying chimeric ARV1::GUS and ARV2::GUS genes showed that ARV gene promoters direct largely overlapping patterns of expression that are restricted to tissues in which cells are actively dividing or expanding. The results of this study support the notion that plants, yeast and mammals share common molecular mechanisms regulating intracellular lipid homeostasis.Elsevier B.V.2026202620062026info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/225881Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.bbalip.2006.03.025Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, 2006, vol. 1761, num.7, p. 725-735https://doi.org/10.1016/j.bbalip.2006.03.025(c) Elsevier B.V., 2006info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2258812026-05-29T05:05:01Z
dc.title.none.fl_str_mv Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
title Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
spellingShingle Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
Forés del Ruste, Oriol
Arabidopsis thaliana
Biologia molecular vegetal
Arabidopsis thaliana
Plant molecular biology
title_short Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
title_full Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
title_fullStr Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
title_full_unstemmed Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
title_sort Arabidopsis thaliana expresses two functional isoforms of Avrp, a protein involved in the regulation of cellular lipid homeostasis
dc.creator.none.fl_str_mv Forés del Ruste, Oriol
Arró i Plans, Montserrat
Pahissa, Albert
Ferrero, Sergi
Germann, Melody
Stukey, Joseph
McDonough, Virgina
Nickels Jr., Joseph T.
Campos Martínez, Narciso
Ferrer i Prats, Albert
author Forés del Ruste, Oriol
author_facet Forés del Ruste, Oriol
Arró i Plans, Montserrat
Pahissa, Albert
Ferrero, Sergi
Germann, Melody
Stukey, Joseph
McDonough, Virgina
Nickels Jr., Joseph T.
Campos Martínez, Narciso
Ferrer i Prats, Albert
author_role author
author2 Arró i Plans, Montserrat
Pahissa, Albert
Ferrero, Sergi
Germann, Melody
Stukey, Joseph
McDonough, Virgina
Nickels Jr., Joseph T.
Campos Martínez, Narciso
Ferrer i Prats, Albert
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Biologia molecular vegetal
Arabidopsis thaliana
Plant molecular biology
topic Arabidopsis thaliana
Biologia molecular vegetal
Arabidopsis thaliana
Plant molecular biology
description Arv1p is involved in the regulation of cellular lipid homeostasis in the yeast Saccharomyces cerevisiae. Here, we report the characterization of the two Arabidopsis thaliana ARV genes and the encoded proteins, AtArv1p and AtArv2p. The functional identity of AtArv1p and AtArv2p was demonstrated by complementation of the thermosensitive phenotype of the arv1Δ yeast mutant strain YJN1756. Both A. thaliana proteins contain the bipartite Arv1 homology domain (AHD), which consists of an NH2-terminal cysteine-rich subdomain with a putative zinc-binding motif followed by a C-terminal subdomain of 33 amino acids. Removal of the cysteine-rich subdomain has no effect on Arvp activity, whereas the presence of the C-terminal subdomain of the AHD is critical for Arvp function. Localization experiments of AtArv1p and AtArv2p tagged with green fluorescent protein (GFP) and expressed in onion epidermal cells demonstrated that both proteins are exclusively targeted to the endoplasmic reticulum. Analysis of β-glucuronidase (GUS) activity in transgenic A. thaliana plants carrying chimeric ARV1::GUS and ARV2::GUS genes showed that ARV gene promoters direct largely overlapping patterns of expression that are restricted to tissues in which cells are actively dividing or expanding. The results of this study support the notion that plants, yeast and mammals share common molecular mechanisms regulating intracellular lipid homeostasis.
publishDate 2006
dc.date.none.fl_str_mv 2006
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225881
url https://hdl.handle.net/2445/225881
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.bbalip.2006.03.025
Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, 2006, vol. 1761, num.7, p. 725-735
https://doi.org/10.1016/j.bbalip.2006.03.025
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2006
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2006
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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