Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity

10 páginas, 4 figuras, 4 tablas

Bibliographic Details
Authors: Moreira, Rebeca, Balseiro, P., Romero Jódar, Alejandro, Dios, S., Posada, David, Novoa, Beatriz, Figueras Huerta, Antonio
Format: article
Publication Date:2012
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/50217
Online Access:http://hdl.handle.net/10261/50217
Access Level:Open access
Keyword:Ruditapes decussatus
Ruditapes philippinarum
Vibrio alginolyticus
EST
Immune-related genes
Gene expression
qPCR
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spelling Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunityMoreira, RebecaBalseiro, P.Romero Jódar, AlejandroDios, S.Posada, DavidNovoa, BeatrizFigueras Huerta, AntonioRuditapes decussatusRuditapes philippinarumVibrio alginolyticusESTImmune-related genesGene expressionqPCR10 páginas, 4 figuras, 4 tablasThe carpet shell clam (Ruditapes decussatus) and Manila clam (Ruditapes philippinarum), which are cultured bivalve species with important commercial value, are affected by diseases that result in large economic losses. Because the molecular mechanism of the immune response of bivalves, especially clams, is scarce and fragmentary, we have examined all Expressed Sequence Tags (EST) resources available in public databases for these two species in order to increase our knowledge on genes related with the immune function in these animals. After automatic annotation and classification of the 3784 not-annotated ESTs of R. decussatus and 4607 of R. philippinarum found in GenBank, 424 ESTs of R. decussatus and 464 of R. philippinarum were found to be putatively involved in immune response. These were carefully reviewed and reannotated. As a result, 13 immune-related ESTs were selected and studied to compare the immune response of R. decussatus and R. philippinarum following a Vibrio alginolyticus challenge. Quantitative PCR was performed, and the expression of each EST was determined. The results showed that, in R. philippinarum, the immune response seems to be faster than that in R. decussatus. Additionally, expression of NF-κB activating genes in R. decussatus did not seem to be sufficient to promote an immune response after Vibrio infection. R. philippinarum, however, was able to trigger and efficiently regulate the transcriptional activity of NF-κB, even when low expression values were reportedThis work has been funded by the Spanish Ministerio de Ciencia e Innovación (MICINN) (AGL2008-05111). R.M. wishes to acknowledge additional funding from the Spanish MICINN through a FPI Spanish research Grant (BES-2009-029765).Peer reviewedElsevier201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/50217reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.dci.2011.06.012info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/502172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
title Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
spellingShingle Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
Moreira, Rebeca
Ruditapes decussatus
Ruditapes philippinarum
Vibrio alginolyticus
EST
Immune-related genes
Gene expression
qPCR
title_short Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
title_full Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
title_fullStr Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
title_full_unstemmed Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
title_sort Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity
dc.creator.none.fl_str_mv Moreira, Rebeca
Balseiro, P.
Romero Jódar, Alejandro
Dios, S.
Posada, David
Novoa, Beatriz
Figueras Huerta, Antonio
author Moreira, Rebeca
author_facet Moreira, Rebeca
Balseiro, P.
Romero Jódar, Alejandro
Dios, S.
Posada, David
Novoa, Beatriz
Figueras Huerta, Antonio
author_role author
author2 Balseiro, P.
Romero Jódar, Alejandro
Dios, S.
Posada, David
Novoa, Beatriz
Figueras Huerta, Antonio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ruditapes decussatus
Ruditapes philippinarum
Vibrio alginolyticus
EST
Immune-related genes
Gene expression
qPCR
topic Ruditapes decussatus
Ruditapes philippinarum
Vibrio alginolyticus
EST
Immune-related genes
Gene expression
qPCR
description 10 páginas, 4 figuras, 4 tablas
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/50217
url http://hdl.handle.net/10261/50217
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.dci.2011.06.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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