Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases

10 p.-4 fig.-2 tab.

Detalhes bibliográficos
Autores: Ferrero, M. L., Torre, Consuelo de la
Formato: artículo
Fecha de publicación:1986
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/245832
Acesso em linha:http://hdl.handle.net/10261/245832
Access Level:acceso abierto
Palavra-chave:8-hydroxyquinoline
Cell cycle
Cell growth
Onion meristems
DNA synthesis
RNA synthesis
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spelling Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerasesFerrero, M. L.Torre, Consuelo de la8-hydroxyquinolineCell cycleCell growthOnion meristemsDNA synthesisRNA synthesis10 p.-4 fig.-2 tab.8-Hydroxyquinoline (HQ) chelates Mg2+ and Mn2+ and, secondarily, affects the activities of DNA and RNA polymerases. The in vivo effect of HQ has been estimated in Allium cepa L. meristems growing under new growth kinetics in the presence of this agent. HQ (at both 5 X 10(-5) M and 10(-4) M) depressed incorporation of [3H]uridine much more effectively than that of [3H]-thymidine. Cycle kinetics in meristems behaved as if they were independent of the rates of synthesis or accumulation of RNA since, under HQ, cycle time was only moderately modified and the new cycle kinetics achieved could be explained by the new rates of [3H]thymidine incorporation. Lengthened S periods were partially compensated for by shortened G2 phases, suggesting that, in these cells, both the growth cycle and its coupling with the DNA-division cycle were not disturbed by a decreased amount of RNA. Finally, the nucleolar cycle during mitosis, but not the interphase nucleolus, was modified under the new rates of RNA synthesis.The work has been partially supported by the 'Comision Asesora de Investigacion Cientffica y Tecnica', Spain. We greatly appreciate the skilful technical and secretarial assistance of Miss M. Carrascosa and Mrs O. Partearroyo.Company of BiologistsComisión Asesora de Investigación Científica y Técnica, CAICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202119862021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/245832reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1242/jcs.80.1.171Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2458322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
title Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
spellingShingle Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
Ferrero, M. L.
8-hydroxyquinoline
Cell cycle
Cell growth
Onion meristems
DNA synthesis
RNA synthesis
title_short Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
title_full Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
title_fullStr Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
title_full_unstemmed Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
title_sort Cell proliferation in Allium cepa L. meristems under 8-hydroxyquinoline, a chelating agent that affects DNA and RNA polymerases
dc.creator.none.fl_str_mv Ferrero, M. L.
Torre, Consuelo de la
author Ferrero, M. L.
author_facet Ferrero, M. L.
Torre, Consuelo de la
author_role author
author2 Torre, Consuelo de la
author2_role author
dc.contributor.none.fl_str_mv Comisión Asesora de Investigación Científica y Técnica, CAICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 8-hydroxyquinoline
Cell cycle
Cell growth
Onion meristems
DNA synthesis
RNA synthesis
topic 8-hydroxyquinoline
Cell cycle
Cell growth
Onion meristems
DNA synthesis
RNA synthesis
description 10 p.-4 fig.-2 tab.
publishDate 1986
dc.date.none.fl_str_mv 1986
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/245832
url http://hdl.handle.net/10261/245832
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1242/jcs.80.1.171
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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