Adenosine receptors are the on-and-off switch of astrocytic cannabinoid type 1 (CB1) receptor effect upon synaptic plasticity in the medial prefrontal cortex

The medial prefrontal cortex (mPFC) is involved in cognitive functions such as work- ing memory. Astrocytic cannabinoid type 1 receptor (CB1R) induces cytosolic calcium (Ca2+) concentration changes with an impact on neuronal function. mPFC astrocytes also express adenosine A1 and A2A receptors (A1R,...

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Detalles Bibliográficos
Autores: Gonçalves-Ribeiro, Joana, Savchak, Oksana K., Costa-Pinto, Sara, Gomes, Joana I., Rivas‐Santisteban, Rafael, Lillo, Alejandro, Sánchez Romero, Javier, Sebastião, Ana M., Navarrete, Marta, Navarro Brugal, Gemma, Franco Fernández, Rafael, Vaz, Sandra H.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/218451
Acceso en línea:https://hdl.handle.net/2445/218451
Access Level:acceso abierto
Palabra clave:Adenosina
Astròcits
Adenosine
Astrocytes
Descripción
Sumario:The medial prefrontal cortex (mPFC) is involved in cognitive functions such as work- ing memory. Astrocytic cannabinoid type 1 receptor (CB1R) induces cytosolic calcium (Ca2+) concentration changes with an impact on neuronal function. mPFC astrocytes also express adenosine A1 and A2A receptors (A1R, A2AR), being unknown the cross- talk between CB1R and adenosine receptors in these cells. We show here that a fur- ther level of regulation of astrocyte Ca2+ signaling occurs through CB1R-A2AR or CB1R-A1R heteromers that ultimately impact mPFC synaptic plasticity. CB1R- mediated Ca2+ transients increased and decreased when A1R and A2AR were acti- vated, respectively, unveiling adenosine receptors as modulators of astrocytic CB1R. CB1R activation leads to an enhancement of long-term potentiation (LTP) in the mPFC, under the control of A1R but not of A2AR. Notably, in IP3R2KO mice, that do not show astrocytic Ca2+ level elevations, CB1R activation decreases LTP, which is not modified by A1R or A2AR. The present work suggests that CB1R has a homeo- static role on mPFC LTP, under the control of A1R, probably due to physical crosstalk between these receptors in astrocytes that ultimately alters CB1R Ca2+ signaling.

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