A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampere...

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Autores: Jeremiasse, Bernadette, Ineveld, Ravian L. van, Bok, Veerle, Kleinnijenhuis, Michiel, Blank, Sam de, Alieva, María, Johnson, Hannah R., Vlie, Esmée J. van, Zeeman, Amber L., Wellens, Lianne M., Llibre-Palomar, Gerard, Barrera Román, Mario, Maggio, Alessia Di, Dekkers, Johanna F., Oliveira, Sabrina, Vahrmeijer, Alexander L., Molenaar, Jan J., Wijnen, Marc HWA, Steeg, Alida FW van der, Wehrens, Ellen J., Ríos, Anne C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/382720
Acceso en línea:http://hdl.handle.net/10261/382720
Access Level:acceso abierto
Palabra clave:Fluorescence-guided Surgery
Patient-derived Organoids
Multi-spectral 3D Imaging
Neuroblastoma
Breast Cancer
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spelling A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgeryJeremiasse, BernadetteIneveld, Ravian L. vanBok, VeerleKleinnijenhuis, MichielBlank, Sam deAlieva, MaríaJohnson, Hannah R.Vlie, Esmée J. vanZeeman, Amber L.Wellens, Lianne M.Llibre-Palomar, GerardBarrera Román, MarioMaggio, Alessia DiDekkers, Johanna F.Oliveira, SabrinaVahrmeijer, Alexander L.Molenaar, Jan J.Wijnen, Marc HWASteeg, Alida FW van derWehrens, Ellen J.Ríos, Anne C.Fluorescence-guided SurgeryPatient-derived OrganoidsMulti-spectral 3D ImagingNeuroblastomaBreast CancerAchieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.This work was financially supported by the Princess Máxima Center for Pediatric Oncology, Oncode Institute, the Netherlands, and the Dutch Neuroblastoma Foundation (Villa Joep). ACR was supported by an European Research Council (ERC)-starting grant 2018 project (no. 804412).Peer reviewedSpringerEMBO PressPrincess Máxima Center for Pediatric OncologyFundación NeuroblastomaEuropean Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/382720reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1038/s44321-024-00084-4https://doi.org/10.1038/s44321-024-00084-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3827202026-05-22T06:33:51Z
dc.title.none.fl_str_mv A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
title A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
spellingShingle A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
Jeremiasse, Bernadette
Fluorescence-guided Surgery
Patient-derived Organoids
Multi-spectral 3D Imaging
Neuroblastoma
Breast Cancer
title_short A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
title_full A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
title_fullStr A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
title_full_unstemmed A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
title_sort A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
dc.creator.none.fl_str_mv Jeremiasse, Bernadette
Ineveld, Ravian L. van
Bok, Veerle
Kleinnijenhuis, Michiel
Blank, Sam de
Alieva, María
Johnson, Hannah R.
Vlie, Esmée J. van
Zeeman, Amber L.
Wellens, Lianne M.
Llibre-Palomar, Gerard
Barrera Román, Mario
Maggio, Alessia Di
Dekkers, Johanna F.
Oliveira, Sabrina
Vahrmeijer, Alexander L.
Molenaar, Jan J.
Wijnen, Marc HWA
Steeg, Alida FW van der
Wehrens, Ellen J.
Ríos, Anne C.
author Jeremiasse, Bernadette
author_facet Jeremiasse, Bernadette
Ineveld, Ravian L. van
Bok, Veerle
Kleinnijenhuis, Michiel
Blank, Sam de
Alieva, María
Johnson, Hannah R.
Vlie, Esmée J. van
Zeeman, Amber L.
Wellens, Lianne M.
Llibre-Palomar, Gerard
Barrera Román, Mario
Maggio, Alessia Di
Dekkers, Johanna F.
Oliveira, Sabrina
Vahrmeijer, Alexander L.
Molenaar, Jan J.
Wijnen, Marc HWA
Steeg, Alida FW van der
Wehrens, Ellen J.
Ríos, Anne C.
author_role author
author2 Ineveld, Ravian L. van
Bok, Veerle
Kleinnijenhuis, Michiel
Blank, Sam de
Alieva, María
Johnson, Hannah R.
Vlie, Esmée J. van
Zeeman, Amber L.
Wellens, Lianne M.
Llibre-Palomar, Gerard
Barrera Román, Mario
Maggio, Alessia Di
Dekkers, Johanna F.
Oliveira, Sabrina
Vahrmeijer, Alexander L.
Molenaar, Jan J.
Wijnen, Marc HWA
Steeg, Alida FW van der
Wehrens, Ellen J.
Ríos, Anne C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Princess Máxima Center for Pediatric Oncology
Fundación Neuroblastoma
European Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fluorescence-guided Surgery
Patient-derived Organoids
Multi-spectral 3D Imaging
Neuroblastoma
Breast Cancer
topic Fluorescence-guided Surgery
Patient-derived Organoids
Multi-spectral 3D Imaging
Neuroblastoma
Breast Cancer
description Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/382720
url http://hdl.handle.net/10261/382720
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1038/s44321-024-00084-4
https://doi.org/10.1038/s44321-024-00084-4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
EMBO Press
publisher.none.fl_str_mv Springer
EMBO Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404492042076160
score 15,812429

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