Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds

The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here,...

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Autores: Palomeras Pairet, Sònia, Rabionet Díaz, Marc, Ferrer Real, Inés, Sarrats Carbó, Ariadna, Garcia-Romeu, Maria Luisa, Puig i Miquel, Teresa, Ciurana, Quim de
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/12961
Acceso en línea:http://hdl.handle.net/10256/12961
Access Level:acceso abierto
Palabra clave:Mama -- Càncer
Breast -- Cancer
Cèl·lules canceroses
Cancer cells
Materials biomèdics
Biomedical materials
Impressió 3D
Three-dimensional printing
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spelling Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) ScaffoldsPalomeras Pairet, SòniaRabionet Díaz, MarcFerrer Real, InésSarrats Carbó, AriadnaGarcia-Romeu, Maria LuisaPuig i Miquel, TeresaCiurana, Quim deMama -- CàncerBreast -- CancerCèl·lules cancerosesCancer cellsMaterials biomèdicsBiomedical materialsImpressió 3DThree-dimensional printingThe cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60° scaffolds were more suitable for 3D culture than the 45° and 90° ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs populationThis work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036)MDPI (Multidisciplinary Digital Publishing Institute)Ministerio de Economía y Competitividad (Espanya)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/12961Molecules, 2016, vol. 21, núm. 4, p. 537Articles publicats (D-CM)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/molecules21040537info:eu-repo/semantics/altIdentifier/eissn/1420-3049info:eu-repo/grantAgreement/MINECO//DPI2013-45201-PAttribution 4.0 Spainhttp://creativecommons.org/licenses/by/4.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/129612026-05-29T05:05:01Z
dc.title.none.fl_str_mv Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
title Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
spellingShingle Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
Palomeras Pairet, Sònia
Mama -- Càncer
Breast -- Cancer
Cèl·lules canceroses
Cancer cells
Materials biomèdics
Biomedical materials
Impressió 3D
Three-dimensional printing
title_short Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
title_full Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
title_fullStr Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
title_full_unstemmed Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
title_sort Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
dc.creator.none.fl_str_mv Palomeras Pairet, Sònia
Rabionet Díaz, Marc
Ferrer Real, Inés
Sarrats Carbó, Ariadna
Garcia-Romeu, Maria Luisa
Puig i Miquel, Teresa
Ciurana, Quim de
author Palomeras Pairet, Sònia
author_facet Palomeras Pairet, Sònia
Rabionet Díaz, Marc
Ferrer Real, Inés
Sarrats Carbó, Ariadna
Garcia-Romeu, Maria Luisa
Puig i Miquel, Teresa
Ciurana, Quim de
author_role author
author2 Rabionet Díaz, Marc
Ferrer Real, Inés
Sarrats Carbó, Ariadna
Garcia-Romeu, Maria Luisa
Puig i Miquel, Teresa
Ciurana, Quim de
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Mama -- Càncer
Breast -- Cancer
Cèl·lules canceroses
Cancer cells
Materials biomèdics
Biomedical materials
Impressió 3D
Three-dimensional printing
topic Mama -- Càncer
Breast -- Cancer
Cèl·lules canceroses
Cancer cells
Materials biomèdics
Biomedical materials
Impressió 3D
Three-dimensional printing
description The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60° scaffolds were more suitable for 3D culture than the 45° and 90° ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs population
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/12961
url http://hdl.handle.net/10256/12961
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules21040537
info:eu-repo/semantics/altIdentifier/eissn/1420-3049
info:eu-repo/grantAgreement/MINECO//DPI2013-45201-P
dc.rights.none.fl_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv Molecules, 2016, vol. 21, núm. 4, p. 537
Articles publicats (D-CM)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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