Membrane promotes tBID interaction with BCL(XL)

9 pages, 6 figures.-- PMID: 19820711 [PubMed].--

Detalles Bibliográficos
Autores: García-Sáez, Ana J., Ries, Jonas, Orzáez, Mar, Pérez-Payá, Enrique, Schwille, Petra
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/17718
Acceso en línea:http://hdl.handle.net/10261/17718
Access Level:acceso abierto
id ES_20be79eefe9eb6d4481e293e26bdec49
oai_identifier_str oai:digital.csic.es:10261/17718
network_acronym_str ES
network_name_str España
repository_id_str
spelling Membrane promotes tBID interaction with BCL(XL)García-Sáez, Ana J.Ries, JonasOrzáez, MarPérez-Payá, EnriqueSchwille, Petra9 pages, 6 figures.-- PMID: 19820711 [PubMed].--Two important questions on the molecular mechanism of the B cell CLL/lymphoma 2 (BCL2) proteins involve the interaction network between pro- and antiapoptotic members and the role of their translocation to the mitochondrial membrane during apoptosis. We used fluorescence correlation spectroscopy to quantify the molecular interactions of BH3-interacting domain death agonist (BID) and its truncated form tBID with the B cell lymphoma extra-large protein truncated at the C terminus (BCL(XL)ΔCt) in solution and in membranes, and we found that (i) only the active form tBID binds to BCL(XL)ΔCt and (ii) that the membrane strongly promotes binding between them. Particularly, a BH3 peptide from BID disrupts the tBID–BCL(XL) complex in solution, but only partially in lipid bilayers. These data indicate that tBID–BCL(XL) interactions in solution and lipid membranes are distinct, and they support a model in which BCL(XL) inhibition of tBID takes place predominantly at the membrane. Our findings imply an active role of the membrane in modulating the interactions between BCL2 proteins that has so far been underestimated.This work was supported by a Marie Curie Intra-European Fellowship (A.J.G.-S.) within the 6th European Framework Program. Work in the E.P.-P. laboratory is supported by the Spanish Ministry of Science and Innovation grants BIO2007-60066 and CSD2008-00005.Peer reviewedNature Publishing Group200920092009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501952582 bytesapplication/pdfhttp://hdl.handle.net/10261/17718reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1038/nsmb.1671info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/177182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Membrane promotes tBID interaction with BCL(XL)
title Membrane promotes tBID interaction with BCL(XL)
spellingShingle Membrane promotes tBID interaction with BCL(XL)
García-Sáez, Ana J.
title_short Membrane promotes tBID interaction with BCL(XL)
title_full Membrane promotes tBID interaction with BCL(XL)
title_fullStr Membrane promotes tBID interaction with BCL(XL)
title_full_unstemmed Membrane promotes tBID interaction with BCL(XL)
title_sort Membrane promotes tBID interaction with BCL(XL)
dc.creator.none.fl_str_mv García-Sáez, Ana J.
Ries, Jonas
Orzáez, Mar
Pérez-Payá, Enrique
Schwille, Petra
author García-Sáez, Ana J.
author_facet García-Sáez, Ana J.
Ries, Jonas
Orzáez, Mar
Pérez-Payá, Enrique
Schwille, Petra
author_role author
author2 Ries, Jonas
Orzáez, Mar
Pérez-Payá, Enrique
Schwille, Petra
author2_role author
author
author
author
description 9 pages, 6 figures.-- PMID: 19820711 [PubMed].--
publishDate 2009
dc.date.none.fl_str_mv 2009
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/17718
url http://hdl.handle.net/10261/17718
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1038/nsmb.1671
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 952582 bytes
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404476561948672
score 15.812429

Similares en LA Referencia