Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity

Ralstonia solanacearum is a bacterial plant pathogen causing important economic losses worldwide. In addition to the polar flagella responsible for swimming motility, this pathogen produces type IV pili (TFP) that govern twitching motility, a flagellum-independent movement on solid surfaces. The imp...

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Autores: Corral, Jordi, Sebastia, Pau, Coll, Núria S., Barbe, Jordi, Aranda, Jesus, Valls i Matheu, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/175115
Acceso en línea:https://hdl.handle.net/2445/175115
Access Level:acceso abierto
Palabra clave:Bacteris patògens
Plantes
Pathogenic bacteria
Plants
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oai_identifier_str oai:diposit.ub.edu:2445/175115
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repository_id_str
spelling Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicityCorral, JordiSebastia, PauColl, Núria S.Barbe, JordiAranda, JesusValls i Matheu, MarcBacteris patògensPlantesPathogenic bacteriaPlantsRalstonia solanacearum is a bacterial plant pathogen causing important economic losses worldwide. In addition to the polar flagella responsible for swimming motility, this pathogen produces type IV pili (TFP) that govern twitching motility, a flagellum-independent movement on solid surfaces. The implication of chemotaxis in plant colonization, through the control flagellar rotation by the proteins CheW and CheA, has been previously reported in R. solanacearum. In this work, we have identified in this bacterium homologues of the Pseudomonas aeruginosa pilI and chpA genes, suggested to play roles in TFP-associated motility analogous to those played by the cheW and cheA genes, respectively. We demonstrate that R. solanacearum strains with a deletion of the pilI or the chpA coding region show normal swimming and chemotaxis but altered biofilm formation and reduced twitching motility, transformation efficiency, and root attachment. Furthermore, these mutants displayed wild-type growth in planta and impaired virulence on tomato plants after soil-drench inoculations but not when directly applied to the xylem. Comparison with deletion mutants for pilA and fliC encoding the major pilin and flagellin subunits, respectively showed that both twitching and swimming are required for plant colonization and full virulence. This work proves for the first time the functionality of a pilus-mediated pathway encoded by pil-chp genes in R. solanacearum, demonstrating that pilI and chpA genes are bona fide motility regulators controlling twitching motility and its three related phenotypes: virulence, natural transformation, and biofilm formation.American Society for Microbiology2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/175115Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1128/mSphere.00740-19mSphere, 2020, vol. 5, num. 2https://doi.org/10.1128/mSphere.00740-19info:eu-repo/grantAgreement/EC/H2020/713673cc-by (c) Corral, Jordi et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1751152026-05-27T06:46:51Z
dc.title.none.fl_str_mv Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
title Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
spellingShingle Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
Corral, Jordi
Bacteris patògens
Plantes
Pathogenic bacteria
Plants
title_short Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
title_full Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
title_fullStr Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
title_full_unstemmed Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
title_sort Twitching and swimming motility play a role in Ralstonia solanacearum pathogenicity
dc.creator.none.fl_str_mv Corral, Jordi
Sebastia, Pau
Coll, Núria S.
Barbe, Jordi
Aranda, Jesus
Valls i Matheu, Marc
author Corral, Jordi
author_facet Corral, Jordi
Sebastia, Pau
Coll, Núria S.
Barbe, Jordi
Aranda, Jesus
Valls i Matheu, Marc
author_role author
author2 Sebastia, Pau
Coll, Núria S.
Barbe, Jordi
Aranda, Jesus
Valls i Matheu, Marc
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacteris patògens
Plantes
Pathogenic bacteria
Plants
topic Bacteris patògens
Plantes
Pathogenic bacteria
Plants
description Ralstonia solanacearum is a bacterial plant pathogen causing important economic losses worldwide. In addition to the polar flagella responsible for swimming motility, this pathogen produces type IV pili (TFP) that govern twitching motility, a flagellum-independent movement on solid surfaces. The implication of chemotaxis in plant colonization, through the control flagellar rotation by the proteins CheW and CheA, has been previously reported in R. solanacearum. In this work, we have identified in this bacterium homologues of the Pseudomonas aeruginosa pilI and chpA genes, suggested to play roles in TFP-associated motility analogous to those played by the cheW and cheA genes, respectively. We demonstrate that R. solanacearum strains with a deletion of the pilI or the chpA coding region show normal swimming and chemotaxis but altered biofilm formation and reduced twitching motility, transformation efficiency, and root attachment. Furthermore, these mutants displayed wild-type growth in planta and impaired virulence on tomato plants after soil-drench inoculations but not when directly applied to the xylem. Comparison with deletion mutants for pilA and fliC encoding the major pilin and flagellin subunits, respectively showed that both twitching and swimming are required for plant colonization and full virulence. This work proves for the first time the functionality of a pilus-mediated pathway encoded by pil-chp genes in R. solanacearum, demonstrating that pilI and chpA genes are bona fide motility regulators controlling twitching motility and its three related phenotypes: virulence, natural transformation, and biofilm formation.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/175115
url https://hdl.handle.net/2445/175115
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1128/mSphere.00740-19
mSphere, 2020, vol. 5, num. 2
https://doi.org/10.1128/mSphere.00740-19
info:eu-repo/grantAgreement/EC/H2020/713673
dc.rights.none.fl_str_mv cc-by (c) Corral, Jordi et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Corral, Jordi et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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