Acrocentric bivalents positioned preferentially nearby to the XY pair in metaphase i human spermatocytes
Objective: To analyze whether the preferential proximity between acrocentric bivalents and the XY pair described at pachytene was maintained in metaphase I human spermatocytes. Design: Proximity frequencies of autosomic bivalents to the sex bivalent were evaluated with the analysis of meiotic prepar...
| Autores: | , , |
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| Formato: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:288869 |
| Acesso em linha: | https://ddd.uab.cat/record/288869 https://dx.doi.org/urn:doi:10.1016/j.fertnstert.2012.07.1110 |
| Access Level: | acceso abierto |
| Palavra-chave: | Acrocentric chromosomes Chromosome territories Human spermatocytes Metaphase I XY bivalent SDG 3 - Good Health and Well-being |
| Resumo: | Objective: To analyze whether the preferential proximity between acrocentric bivalents and the XY pair described at pachytene was maintained in metaphase I human spermatocytes. Design: Proximity frequencies of autosomic bivalents to the sex bivalent were evaluated with the analysis of meiotic preparations combining sequentially standard techniques and multiplex fluorescence in situ hybridization. Setting: Assisted reproduction centers. Patient(s): Thirty-seven men consulting for fertility problems. Intervention(s): Unilateral testicular biopsies. Main Outcome Measure(s): Proximity frequencies analysis to the XY pair, evaluated individually and grouping bivalents, was carried out using a logistical regression model with repeated measures. Result(s): Bivalents 22 and 15 were observed more frequently near to the sex bivalent than the others. Significant interindividual differences were not observed. Conclusion(s): Results suggest that bivalents distribution to the metaphase plate is nonrandom. The maintenance of the acrocentric chromosomes' proximity to the sex bivalent from pachytene to metaphase I would indicate that the relative bivalents position would be notably preserved. The observation of non-interindividual variability, despite different infertility etiology, suggests that the nuclear organization pattern remains largely unaffected even if spermatogenesis is compromised. |
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