Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit,

This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency no...

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Detalles Bibliográficos
Autores: García-Martinez, Gonzalo, Alonso Bustabad, Enrique, Perrot, Hubert, Gabrielli, Claude, Bucur, Bogdan, Lazerges, Mathieu, Rose, Daniel, Rodriguez-Pardo, Loreto, Fariña Tojo, Jose, Compère, Chantal, Arnau Vives, Antonio|||0000-0002-5709-1690
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/28713
Acceso en línea:https://riunet.upv.es/handle/10251/28713
Access Level:acceso abierto
Palabra clave:DNA sequence detection
Electronic oscillator
Frequency noise
Quartz crystal microbalance
Resolution
Sensitivity
Disulfide
DNA
Article
Calibration
Chemistry
Electrochemistry
Electronics
Equipment design
Genetic procedures
Methodology
Oscillometry
Physics
Reproducibility
Biosensing Techniques
Disulfides
Quartz Crystal Microbalance Techniques
Reproducibility of Results
TECNOLOGIA ELECTRONICA
Descripción
Sumario:This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm 2 in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected. © 2011 by the authors; licensee MDPI, Basel, Switzerland.

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