Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids

Supramolecular hydrogels based on N-protected phenylalanine (Fmoc–Phe–OH) were used to encapsulate non-ionic surfactant vesicles (niosomes). The niosomes consisted of an amphiphilic lipid mixed with polysorbate-80 and electrostatically complexed with a fluorescently labelled oligodeoxynucleotide (FI...

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Autores: Grijalvo, Santiago, Puras, Gustavo, Zárate, Jon, Pons Pons, Ramon, Pedraz, José Luís, Eritja Casadellà, Ramón, Díaz Díaz, David
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/132676
Acceso en línea:http://hdl.handle.net/10261/132676
Access Level:acceso abierto
Palabra clave:Supramolecular hydrogels
Nioplexes
Supramolecular hybrid biohydrogels
Nucleic acids
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spelling Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acidsGrijalvo, SantiagoPuras, GustavoZárate, JonPons Pons, RamonPedraz, José LuísEritja Casadellà, RamónDíaz Díaz, DavidSupramolecular hydrogelsNioplexesSupramolecular hybrid biohydrogelsNucleic acidsSupramolecular hydrogels based on N-protected phenylalanine (Fmoc–Phe–OH) were used to encapsulate non-ionic surfactant vesicles (niosomes). The niosomes consisted of an amphiphilic lipid mixed with polysorbate-80 and electrostatically complexed with a fluorescently labelled oligodeoxynucleotide (FITC–ODN) as a model nucleic acid derivative. The diffusion properties of the supramolecular hydrogel were conveniently tuned by adding a small amount of κ-carrageenan (≤1% w/v) as a crosslinking agent. Interestingly, neither cationic niosomes nor the biopolymer additive significantly affected the hydrogelation properties of the amino acid-based low molecular weight (LMW) gelator. In vitro drug release experiments from Fmoc–Phe–OH hydrogels containing cationic niosomes were successfully carried out in the absence and in the presence of κ-carrageenan. The niosomal ODN liberation in solution was fitted using Higuchi, Korsmeyer–Peppas and Weibull drug release models, showing the prevalence of diffusion mechanisms in each case. Moreover, the time release was easily prolonged by increasing the concentration of κ-carrageenan. Preliminary transfection studies indicate the suitability of these supramolecular hybrid hydrogels to embed niosomal formulations and, consequently, for being used as tunable delivery vehicles for nucleic acids.This work was supported by the Spanish Ministry of Education (Grant CTQ2013-41514- P, CTQ2014-52588-R, RTC-2014-2038-1), the University of Regensburg, the DFG (DI 1748/3-1), the Generalitat de Catalunya (2014/SGR/624) and the Instituto de Salud Carlos III (CB06_01_0019). S.G. thanks the Spanish Ministry of Education, Culture and Sports for a “Jose Castillejo” grant within the researcher mobility program (Programa Estatal de Promoción del Talento y su Empleabilidad en I+D+i, Subprograma Estatal de Movilidad, del Plan Estatal de Investigación Científica y Técnica y de Innovación 2013- 2016). D.D.D. thanks the Deutsche Forschungsgemeinschaft (DFG) for the Heisenberg Professorship Award. We thank the Göpferich research group for assistance and granting us access to the rheometer. We would like to thank Dr. Gemma Fabriàs and Mireia Casasampere for assistance and access to the cytometer and Dr. Cristina Garrido for her help in the cytometer data processing.Peer reviewedRoyal Society of Chemistry (UK)Ministerio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/132676reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1039/C6RA01005ASíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1326762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
title Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
spellingShingle Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
Grijalvo, Santiago
Supramolecular hydrogels
Nioplexes
Supramolecular hybrid biohydrogels
Nucleic acids
title_short Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
title_full Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
title_fullStr Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
title_full_unstemmed Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
title_sort Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids
dc.creator.none.fl_str_mv Grijalvo, Santiago
Puras, Gustavo
Zárate, Jon
Pons Pons, Ramon
Pedraz, José Luís
Eritja Casadellà, Ramón
Díaz Díaz, David
author Grijalvo, Santiago
author_facet Grijalvo, Santiago
Puras, Gustavo
Zárate, Jon
Pons Pons, Ramon
Pedraz, José Luís
Eritja Casadellà, Ramón
Díaz Díaz, David
author_role author
author2 Puras, Gustavo
Zárate, Jon
Pons Pons, Ramon
Pedraz, José Luís
Eritja Casadellà, Ramón
Díaz Díaz, David
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Supramolecular hydrogels
Nioplexes
Supramolecular hybrid biohydrogels
Nucleic acids
topic Supramolecular hydrogels
Nioplexes
Supramolecular hybrid biohydrogels
Nucleic acids
description Supramolecular hydrogels based on N-protected phenylalanine (Fmoc–Phe–OH) were used to encapsulate non-ionic surfactant vesicles (niosomes). The niosomes consisted of an amphiphilic lipid mixed with polysorbate-80 and electrostatically complexed with a fluorescently labelled oligodeoxynucleotide (FITC–ODN) as a model nucleic acid derivative. The diffusion properties of the supramolecular hydrogel were conveniently tuned by adding a small amount of κ-carrageenan (≤1% w/v) as a crosslinking agent. Interestingly, neither cationic niosomes nor the biopolymer additive significantly affected the hydrogelation properties of the amino acid-based low molecular weight (LMW) gelator. In vitro drug release experiments from Fmoc–Phe–OH hydrogels containing cationic niosomes were successfully carried out in the absence and in the presence of κ-carrageenan. The niosomal ODN liberation in solution was fitted using Higuchi, Korsmeyer–Peppas and Weibull drug release models, showing the prevalence of diffusion mechanisms in each case. Moreover, the time release was easily prolonged by increasing the concentration of κ-carrageenan. Preliminary transfection studies indicate the suitability of these supramolecular hybrid hydrogels to embed niosomal formulations and, consequently, for being used as tunable delivery vehicles for nucleic acids.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/132676
url http://hdl.handle.net/10261/132676
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1039/C6RA01005A
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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