Community recommendations on cryoEM data archiving and validation

In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and arch...

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Detalles Bibliográficos
Autores: Kleywegt, Gerard J., Adams, Paul D., Butcher, Sarah J., Lawson, Catherine L., Rohou, Alexis, Rosenthal, Peter B., Subramaniam, Sriram, Topf, Maya, Abbott, Sanja, Baldwin, Philip R., Berrisford, John M., Bricogne, Gérard, Choudhary, Preeti, Croll, Tristan I., Danev, Radostin, Ganesan, Sai J., Grant, Timothy, Gutmanas, Aleksandras, Henderson, Richard, Heymann, J. Bernard, Huiskonen, Juha T., Istrate, Andrei, Kato, Takayuki, Lander, Gabriel C., Lok, Shee-Mei, Ludtke, Steven J., Murshudov, Garib N., Pye, Ryan, Pintilie, Grigore D., Richardson, Jane S., Sachse, Carsten, Salih, Osman, Scheres, Sjors H. W., Schroeder, Gunnar F., Sorzano, Carlos Óscar S., Stagg, Scott M., Wang, Zhe, Warshamanage, Rangana, Westbrook, John D., Winn, Martyn D., Young, Jasmine Y., Burley, Stephen K., Hoch, Jeffrey C., Kurisu, Genji, Morris, Kyle, Patwardhan, Ardan, Velankar, Sameer
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/358564
Acceso en línea:http://hdl.handle.net/10261/358564
Access Level:acceso abierto
Palabra clave:Cryogenic-specimen electron microscopy
Data archiving
Validation
Quality control
Electron Microscopy Data Bank
Protein Data Bank
Single-particle cryoEM
Databases
Bioinformatics
Structure determination
Descripción
Sumario:In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop’s motivation and history, the topics discussed, and the resulting consensus recommendations. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations.

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