High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem

18 pages, 5 tables, 7 figures.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)

Detalles Bibliográficos
Autores: Ríos-Castro, Raquel, Romero Jódar, Alejandro, Aranguren, Raquel, Pallavicini, Alberto, Banchi, Elisa, Novoa, Beatriz, Figueras Huerta, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/260973
Acceso en línea:http://hdl.handle.net/10261/260973
Access Level:acceso abierto
Palabra clave:Metabarcoding
Eukaryote
High-throughput sequencing
eDNA
Environment
Pathogens
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spelling High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling EcosystemRíos-Castro, RaquelRomero Jódar, AlejandroAranguren, RaquelPallavicini, AlbertoBanchi, ElisaNovoa, BeatrizFigueras Huerta, AntonioMetabarcodingEukaryoteHigh-throughput sequencingeDNAEnvironmentPathogens18 pages, 5 tables, 7 figures.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)The marine environment includes diverse microeukaryotic organisms that play important functional roles in the ecosystem. With molecular approaches, eukaryotic taxonomy has been improved, complementing classical analysis. In this study, DNA metabarcoding was performed to describe putative pathogenic eukaryotic microorganisms in sediment and marine water fractions collected in Galicia (NW Spain) from 2016 to 2018. The composition of eukaryotic communities was distinct between sediment and water fractions. Protists were the most diverse group, with the clade TSAR (Stramenopiles, Alveolata, Rhizaria, and Telonemida) as the primary representative organisms in the environment. Harmful algae and invasive species were frequently detected. Potential pathogens, invasive pathogenic organisms as well as the causative agents of harmful phytoplanktonic blooms were identified in this marine ecosystem. Most of the identified pathogens have a crucial impact on the aquacultural sector or affect to relevant species in the marine ecosystem, such as diatoms. Moreover, pathogens with medical and veterinary importance worldwide were also found, as well as pathogens that affect diatoms. The evaluation of the health of a marine ecosystem that directly affects the aquacultural sector with a zoonotic concern was performed with the metabarcoding assayThis work was conducted with the support of the projects AGL2015-65705-R (Ministerio de Economía y Competitividad, Spain), IN607B 2019/01 (Consellería de Economía, Emprego e Industria–GAIN, Xunta de Galicia), 0474_BLUEBIOLAB Fondo Europeo de Desarrollo Regional FEDER en el marco del programa Interreg V A España – Portugal (POCTEP) 2014-2020, and VIVALDI (678589) (EU H2O20). RR-C wishes to thank the Axencia Galega de Innovación (GAIN, Xunta de Galicia) for her predoctoral contract IN606A-2018/020Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)European CommissionXunta de GaliciaAxencia Galega de InnovaciónConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/260973reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-65705-Rinfo:eu-repo/grantAgreement/EC/H2020/678589The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3389/fvets.2021.765606https://doi.org/10.3389/fvets.2021.765606Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2609732026-05-22T06:33:51Z
dc.title.none.fl_str_mv High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
title High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
spellingShingle High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
Ríos-Castro, Raquel
Metabarcoding
Eukaryote
High-throughput sequencing
eDNA
Environment
Pathogens
title_short High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
title_full High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
title_fullStr High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
title_full_unstemmed High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
title_sort High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem
dc.creator.none.fl_str_mv Ríos-Castro, Raquel
Romero Jódar, Alejandro
Aranguren, Raquel
Pallavicini, Alberto
Banchi, Elisa
Novoa, Beatriz
Figueras Huerta, Antonio
author Ríos-Castro, Raquel
author_facet Ríos-Castro, Raquel
Romero Jódar, Alejandro
Aranguren, Raquel
Pallavicini, Alberto
Banchi, Elisa
Novoa, Beatriz
Figueras Huerta, Antonio
author_role author
author2 Romero Jódar, Alejandro
Aranguren, Raquel
Pallavicini, Alberto
Banchi, Elisa
Novoa, Beatriz
Figueras Huerta, Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Xunta de Galicia
Axencia Galega de Innovación
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Metabarcoding
Eukaryote
High-throughput sequencing
eDNA
Environment
Pathogens
topic Metabarcoding
Eukaryote
High-throughput sequencing
eDNA
Environment
Pathogens
description 18 pages, 5 tables, 7 figures.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/260973
url http://hdl.handle.net/10261/260973
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2015-65705-R
info:eu-repo/grantAgreement/EC/H2020/678589
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3389/fvets.2021.765606
https://doi.org/10.3389/fvets.2021.765606
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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