Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome

The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well docum...

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Authors: Sanchez-Osuna, M, Barbe, J, Erill, I
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Institut d'Investigació i Innovació Parc Taulí (I3PT)
Repository:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
OAI Identifier:oai:i3pt.fundanetsuite.com:p2265
Online Access:https://i3pt.portalinvestigacion.com/publicaciones/2265
Access Level:Open access
Keyword:antimicrobial resistance
dissemination
evolution
plasmid
mobile genetic element
GC content
horizontal gene transfer
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spelling Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global PlasmidomeSanchez-Osuna, MBarbe, JErill, Iantimicrobial resistancedisseminationevolutionplasmidmobile genetic elementGC contenthorizontal gene transferThe emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data.MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://i3pt.portalinvestigacion.com/publicaciones/2265Antibiotics-BaselISSN: 20796382reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulíinstname:Institut d'Investigació i Innovació Parc Taulí (I3PT)Inglésinfo:eu-repo/semantics/openAccessoai:i3pt.fundanetsuite.com:p22652026-06-21T15:30:37Z
dc.title.none.fl_str_mv Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
spellingShingle Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
Sanchez-Osuna, M
antimicrobial resistance
dissemination
evolution
plasmid
mobile genetic element
GC content
horizontal gene transfer
title_short Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_full Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_fullStr Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_full_unstemmed Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
title_sort Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
dc.creator.none.fl_str_mv Sanchez-Osuna, M
Barbe, J
Erill, I
author Sanchez-Osuna, M
author_facet Sanchez-Osuna, M
Barbe, J
Erill, I
author_role author
author2 Barbe, J
Erill, I
author2_role author
author
dc.subject.none.fl_str_mv antimicrobial resistance
dissemination
evolution
plasmid
mobile genetic element
GC content
horizontal gene transfer
topic antimicrobial resistance
dissemination
evolution
plasmid
mobile genetic element
GC content
horizontal gene transfer
description The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://i3pt.portalinvestigacion.com/publicaciones/2265
url https://i3pt.portalinvestigacion.com/publicaciones/2265
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Antibiotics-Basel
ISSN: 20796382
reponame:r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
instname:Institut d'Investigació i Innovació Parc Taulí (I3PT)
instname_str Institut d'Investigació i Innovació Parc Taulí (I3PT)
reponame_str r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
collection r-I3PT. Repositorio Institucional Producción Científica del Institut d'Investigació i Innovació Parc Taulí
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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