Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm

The extracellular matrix protects biofilm cells by reducing diffusion of antimicrobials. Tobramycin is an antibiotic used extensively to treat P. aeruginosa biofilms, but it is sequestered in the biofilm periphery by the extracellular negative charge matrix and loses its efficacy significantly. Disp...

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Authors: Blanco-Cabra, Núria, Movellan, Julie, Marradi, Marco, Gracia, Raquel, Salvador, Cristian, Dupin, Damien, Loinaz, Iraida, Torrents Serra, Eduard
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/189104
Online Access:https://hdl.handle.net/2445/189104
Access Level:Open access
Keyword:Biofilms
Medicaments antibacterians
Nanopartícules
Antibacterial agents
Nanoparticles
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spelling Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilmBlanco-Cabra, NúriaMovellan, JulieMarradi, MarcoGracia, RaquelSalvador, CristianDupin, DamienLoinaz, IraidaTorrents Serra, EduardBiofilmsMedicaments antibacteriansNanopartículesBiofilmsAntibacterial agentsNanoparticlesThe extracellular matrix protects biofilm cells by reducing diffusion of antimicrobials. Tobramycin is an antibiotic used extensively to treat P. aeruginosa biofilms, but it is sequestered in the biofilm periphery by the extracellular negative charge matrix and loses its efficacy significantly. Dispersal of the biofilm extracellular matrix with enzymes such as DNase I is another promising therapy that enhances antibiotic diffusion into the biofilm. Here, we combine the charge neutralization of tobramycin provided by dextran-based single-chain polymer nanoparticles (SCPNs) together with DNase I to break the biofilm matrix. Our study demonstrates that the SCPNs improve the activity of tobramycin and DNase I by neutralizing the ionic interactions that keep this antibiotic in the biofilm periphery. Moreover, the detailed effects and interactions of nanoformulations with extracellular matrix components were revealed through time-lapse imaging of the P. aeruginosa biofilms by laser scanning confocal microscopy with specific labeling of the different biofilm components.npj with Nanyang Technological University2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/189104Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41522-022-00317-9Npj Biofilms And Microbiomes, 2022, vol. 8, num. 1, p. 52https://doi.org/10.1038/s41522-022-00317-9cc by (c) Blanco Cabra, Núria et al., 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1891042026-05-27T06:46:51Z
dc.title.none.fl_str_mv Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
title Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
spellingShingle Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
Blanco-Cabra, Núria
Biofilms
Medicaments antibacterians
Nanopartícules
Biofilms
Antibacterial agents
Nanoparticles
title_short Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
title_full Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
title_fullStr Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
title_full_unstemmed Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
title_sort Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
dc.creator.none.fl_str_mv Blanco-Cabra, Núria
Movellan, Julie
Marradi, Marco
Gracia, Raquel
Salvador, Cristian
Dupin, Damien
Loinaz, Iraida
Torrents Serra, Eduard
author Blanco-Cabra, Núria
author_facet Blanco-Cabra, Núria
Movellan, Julie
Marradi, Marco
Gracia, Raquel
Salvador, Cristian
Dupin, Damien
Loinaz, Iraida
Torrents Serra, Eduard
author_role author
author2 Movellan, Julie
Marradi, Marco
Gracia, Raquel
Salvador, Cristian
Dupin, Damien
Loinaz, Iraida
Torrents Serra, Eduard
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biofilms
Medicaments antibacterians
Nanopartícules
Biofilms
Antibacterial agents
Nanoparticles
topic Biofilms
Medicaments antibacterians
Nanopartícules
Biofilms
Antibacterial agents
Nanoparticles
description The extracellular matrix protects biofilm cells by reducing diffusion of antimicrobials. Tobramycin is an antibiotic used extensively to treat P. aeruginosa biofilms, but it is sequestered in the biofilm periphery by the extracellular negative charge matrix and loses its efficacy significantly. Dispersal of the biofilm extracellular matrix with enzymes such as DNase I is another promising therapy that enhances antibiotic diffusion into the biofilm. Here, we combine the charge neutralization of tobramycin provided by dextran-based single-chain polymer nanoparticles (SCPNs) together with DNase I to break the biofilm matrix. Our study demonstrates that the SCPNs improve the activity of tobramycin and DNase I by neutralizing the ionic interactions that keep this antibiotic in the biofilm periphery. Moreover, the detailed effects and interactions of nanoformulations with extracellular matrix components were revealed through time-lapse imaging of the P. aeruginosa biofilms by laser scanning confocal microscopy with specific labeling of the different biofilm components.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/189104
url https://hdl.handle.net/2445/189104
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41522-022-00317-9
Npj Biofilms And Microbiomes, 2022, vol. 8, num. 1, p. 52
https://doi.org/10.1038/s41522-022-00317-9
dc.rights.none.fl_str_mv cc by (c) Blanco Cabra, Núria et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Blanco Cabra, Núria et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv npj with Nanyang Technological University
publisher.none.fl_str_mv npj with Nanyang Technological University
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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