Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana

[EN] Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned a wild ZYMV isolate and inserted the visual marker Roseal to obtain recombinant clone ZYMV-Rosl. While in some plant-virus combinations Roseal induces accumulati...

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Autores: Cordero-Cucart, María Teresa, Cerdán García, Lidia, Katsarou, Konstantina, Kalantidis, Kriton, CARBONELL, ALBERTO|||0000-0001-5628-6632, Daròs, José-Antonio|||0000-0002-6535-2889
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/85239
Acceso en línea:https://riunet.upv.es/handle/10251/85239
Access Level:acceso abierto
Palabra clave:Anthocyanin biosynthesis
Arabidopsis thaliana
Antiviral defense
Cucurbita pepo
Vector system
Protein gene
Plants
Infection
RNA
Expression
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spelling Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamianaCordero-Cucart, María TeresaCerdán García, LidiaKatsarou, KonstantinaKalantidis, KritonCARBONELL, ALBERTO|||0000-0001-5628-6632Daròs, José-Antonio|||0000-0002-6535-2889Anthocyanin biosynthesisArabidopsis thalianaAntiviral defenseCucurbita pepoVector systemProtein genePlantsInfectionRNAExpression[EN] Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned a wild ZYMV isolate and inserted the visual marker Roseal to obtain recombinant clone ZYMV-Rosl. While in some plant-virus combinations Roseal induces accumulation of anthocyanins in infected tissues, ZYMV-Rosl infection of cucurbits did not lead to detectable anthocyanin accumulation. However, the recombinant virus did induce dark red pigmen-tation in infected tissues of the model plant Nicotiana ben-thamiana. In this species, ZYMV-Rosl multiplied efficiently in local inoculated tissue but only a few progeny particles estab-lished infection foci in upper leaves. We used this system to analyze the roles of Dicer-like (DCL) genes, core components of plant antiviral RNA silencing pathways, in ZYMV infection. ZYMV-Rosl local replication was not significantly affected in single DCL knockdown lines nor in double DCL2/4 and triple DCL2/3/4 knockdown lines. ZYMV-Rosl systemic accumula-tion was not affected in knockdown lines DCL1, DCL2, and DCL3. However in DCL4 and also in DCL2/4 and DCL2/3/4 knockdown lines, ZYMV-Rosl systemic accumulation dra-matically increased, which highlights the key role of DCL4 in restricting virus systemic movement. The effect of DCL4 on ZYMV systemic movement was confirmed with a wild-type version of the virus.We thank V. Aragones for excellent technical assistance. This work was supported by the Spanish Ministerio de Economia y Competitividad (MINECO) through grants BI02014-54269-R and AGL2013-49919-EXP and by the Greek Ministry for Education and Religious Affairs (Program Aristeia II, 4499, ViroidmiR; ESPA 2007-2013). A. Carbonell was supported by an Individual Fellowship from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 655841.American Phytopathological SocietyInstituto Universitario Mixto de Biología Molecular y Celular de PlantasEuropean CommissionMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/85239reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 H2020 655841 Genome-wide analysis of RNA and protein interacting profiles during a plant virus infectionMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-54269-R INSTRUMENTOS BIOTECNOLOGICOS DERIVADOS DE VIRUS DE PLANTASMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2013-49919-EXP DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTASopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/852392026-06-13T07:49:27Z
dc.title.none.fl_str_mv Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
title Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
spellingShingle Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
Cordero-Cucart, María Teresa
Anthocyanin biosynthesis
Arabidopsis thaliana
Antiviral defense
Cucurbita pepo
Vector system
Protein gene
Plants
Infection
RNA
Expression
title_short Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
title_full Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
title_fullStr Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
title_full_unstemmed Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
title_sort Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana
dc.creator.none.fl_str_mv Cordero-Cucart, María Teresa
Cerdán García, Lidia
Katsarou, Konstantina
Kalantidis, Kriton
CARBONELL, ALBERTO|||0000-0001-5628-6632
Daròs, José-Antonio|||0000-0002-6535-2889
author Cordero-Cucart, María Teresa
author_facet Cordero-Cucart, María Teresa
Cerdán García, Lidia
Katsarou, Konstantina
Kalantidis, Kriton
CARBONELL, ALBERTO|||0000-0001-5628-6632
Daròs, José-Antonio|||0000-0002-6535-2889
author_role author
author2 Cerdán García, Lidia
Katsarou, Konstantina
Kalantidis, Kriton
CARBONELL, ALBERTO|||0000-0001-5628-6632
Daròs, José-Antonio|||0000-0002-6535-2889
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
European Commission
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Anthocyanin biosynthesis
Arabidopsis thaliana
Antiviral defense
Cucurbita pepo
Vector system
Protein gene
Plants
Infection
RNA
Expression
topic Anthocyanin biosynthesis
Arabidopsis thaliana
Antiviral defense
Cucurbita pepo
Vector system
Protein gene
Plants
Infection
RNA
Expression
description [EN] Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned a wild ZYMV isolate and inserted the visual marker Roseal to obtain recombinant clone ZYMV-Rosl. While in some plant-virus combinations Roseal induces accumulation of anthocyanins in infected tissues, ZYMV-Rosl infection of cucurbits did not lead to detectable anthocyanin accumulation. However, the recombinant virus did induce dark red pigmen-tation in infected tissues of the model plant Nicotiana ben-thamiana. In this species, ZYMV-Rosl multiplied efficiently in local inoculated tissue but only a few progeny particles estab-lished infection foci in upper leaves. We used this system to analyze the roles of Dicer-like (DCL) genes, core components of plant antiviral RNA silencing pathways, in ZYMV infection. ZYMV-Rosl local replication was not significantly affected in single DCL knockdown lines nor in double DCL2/4 and triple DCL2/3/4 knockdown lines. ZYMV-Rosl systemic accumula-tion was not affected in knockdown lines DCL1, DCL2, and DCL3. However in DCL4 and also in DCL2/4 and DCL2/3/4 knockdown lines, ZYMV-Rosl systemic accumulation dra-matically increased, which highlights the key role of DCL4 in restricting virus systemic movement. The effect of DCL4 on ZYMV systemic movement was confirmed with a wild-type version of the virus.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/85239
url https://riunet.upv.es/handle/10251/85239
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 H2020 655841 Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2014-54269-R INSTRUMENTOS BIOTECNOLOGICOS DERIVADOS DE VIRUS DE PLANTAS
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2013-49919-EXP DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTAS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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