The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties

© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Detalles Bibliográficos
Autores: Ballesteros, Uxue, Iriondo, Marina N., Varela, Yaiza R., Goñi, Félix M., Alonso, Alicia, Montes, L. Ruth, Etxaniz, Asier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/357343
Acceso en línea:http://hdl.handle.net/10261/357343
Access Level:acceso abierto
Palabra clave:Autophagy
LC3C
Membrane fusion
ATG8
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spelling The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion propertiesBallesteros, UxueIriondo, Marina N.Varela, Yaiza R.Goñi, Félix M.Alonso, AliciaMontes, L. RuthEtxaniz, AsierAutophagyLC3CMembrane fusionATG8© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Autophagy is a catabolic process in which a double-membrane organelle, the autophagosome (AP), engulfs cellular components that will be degraded in the lysosomes. ATG8 protein family members participate at various stages of AP formation. The present study compares the capacity to induce lipid-vesicle tethering and fusion of two ATG8 family members, LC3B and LC3C, with model membranes. LC3B is the most thoroughly studied ATG8 protein. It is generally considered as an autophagosomal marker and a canonical representative of the LC3 subfamily. LC3C is less studied, but recent data have reported its implication in various processes, crucial to cellular homeostasis. The results in this paper show that LC3C induces higher levels of tethering and of intervesicular lipid mixing than LC3B. As the N-terminus of LC3C is different from that of the other family members, various mutants of the N-terminal region of both LC3B and LC3C were designed, and their activities compared. It was concluded that the N-terminal region of LC3C was responsible for the enhanced vesicle tethering, membrane perturbation and vesicle-vesicle fusion activities of LC3C as compared to LC3B. The results suggest a specialized function of LC3C in the AP expansion process.This work was funded in part by the Spanish Ministry of Science, Innovation, and Universities (MCIU), Agencia Estatal de Investigación (AEI), Fondo Europeo de Desarrollo Regional (FEDER) (grant No. PID2021-124461NB-I00), the Basque Government (grant No. IT1625-22), Fundación Biofísica Bizkaia, and the Basque Excellence Research Centre (BERC) program of the Basque Government. Y.R.V. was a recipient of a pre-doctoral FPU fellowship from the Spanish Ministry of Science, Innovation, and Universities (FPU18/00799). U.B. thanks the University of the Basque Country for a pre-doctoral contract (PIF19-152).Peer reviewedElsevier BVMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionEusko JaurlaritzaFundación Biofísica BizkaiaBasque Excellence Research CentreBallesteros, Uxue [0000-0001-8166-6485]Iriondo, Marina N. [0000-0002-3816-0865]Varela, Yaiza R. [0000-0002-7601-4025]Goñi, Félix M. [0000-0001-6270-9216]Alonso, Alicia [0000-0002-2730-7470]Montes, L. Ruth [0000-0002-4766-8417]Etxaniz, Asier [0000-0003-2916-8256]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/357343reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124461NB-I00https://doi.org/10.1016/j.ijbiomac.2024.129835Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3573432026-05-22T06:33:51Z
dc.title.none.fl_str_mv The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
title The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
spellingShingle The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
Ballesteros, Uxue
Autophagy
LC3C
Membrane fusion
ATG8
title_short The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
title_full The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
title_fullStr The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
title_full_unstemmed The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
title_sort The N-terminal region of the ATG8 autophagy protein LC3C is essential for its membrane fusion properties
dc.creator.none.fl_str_mv Ballesteros, Uxue
Iriondo, Marina N.
Varela, Yaiza R.
Goñi, Félix M.
Alonso, Alicia
Montes, L. Ruth
Etxaniz, Asier
author Ballesteros, Uxue
author_facet Ballesteros, Uxue
Iriondo, Marina N.
Varela, Yaiza R.
Goñi, Félix M.
Alonso, Alicia
Montes, L. Ruth
Etxaniz, Asier
author_role author
author2 Iriondo, Marina N.
Varela, Yaiza R.
Goñi, Félix M.
Alonso, Alicia
Montes, L. Ruth
Etxaniz, Asier
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Eusko Jaurlaritza
Fundación Biofísica Bizkaia
Basque Excellence Research Centre
Ballesteros, Uxue [0000-0001-8166-6485]
Iriondo, Marina N. [0000-0002-3816-0865]
Varela, Yaiza R. [0000-0002-7601-4025]
Goñi, Félix M. [0000-0001-6270-9216]
Alonso, Alicia [0000-0002-2730-7470]
Montes, L. Ruth [0000-0002-4766-8417]
Etxaniz, Asier [0000-0003-2916-8256]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Autophagy
LC3C
Membrane fusion
ATG8
topic Autophagy
LC3C
Membrane fusion
ATG8
description © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/357343
url http://hdl.handle.net/10261/357343
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124461NB-I00
https://doi.org/10.1016/j.ijbiomac.2024.129835
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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