Identification of a crucial INO2 allele for enhancing ethanol resistance in an industrial fermentation strain of Saccharomyces cerevisiae
Ethanol toxicity is a major challenge for Saccharomyces cerevisiae during fermentation, affecting its growth and influencing the process. This study investigated the molecular mechanisms of ethanol tolerance using transcriptomics analysis of three S. cerevisiae strains chosen due to their differing...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/405793 |
| Acceso en línea: | http://hdl.handle.net/10261/405793 |
| Access Level: | acceso abierto |
| Palabra clave: | Saccharomyces cerevisiae Ethanol stress Transcriptomics Membrane lipids Inositol transcriptomics |
| Sumario: | Ethanol toxicity is a major challenge for Saccharomyces cerevisiae during fermentation, affecting its growth and influencing the process. This study investigated the molecular mechanisms of ethanol tolerance using transcriptomics analysis of three S. cerevisiae strains chosen due to their differing levels of resistance to ethanol described in a previous work, which linked them to differences in their membrane compositions. Transcriptomic analysis revealed distinct responses in membrane lipid synthesis genes, particularly those involved in ergosterol biosynthesis, in ethanol-tolerant strains carrying a variant of the INO2 allele. This variant, which includes V263I and H86R amino acid replacements in the Ino2p transcription factor, was exclusive to ethanol-tolerant strains. CRISPR-Cas9-mediated reversion of the variant INO2 allele to the wild-type sequence in the highly tolerant strain AJ4 resulted in decreased ethanol tolerance. Our findings demonstrate the crucial role of Ino2p in ethanol tolerance through its regulation of lipid synthesis and membrane composition, highlighting the complex interplay of transcription factors in strain-specific ethanol resistance. |
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