Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs

Background The composition and distribution of fatty acids (FA) are important factors determining the quality, flavor, and nutrient value of meat. In addition, FAs synthesized in the body participate in energy metabolism and are involved in different regulatory pathways in the form of signaling mole...

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Autores: Liu, Junhui, Sebastià, Cristina, JOVÉ JUNCÀ, TEODOR, Quintanilla, Raquel, González-Rodríguez, Olga, Passols, Magí, Castelló, Anna, Sánchez, Armand, Ballester Devis, Maria, Folch, Josep M.
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/3596
Acceso en línea:http://hdl.handle.net/20.500.12327/3596
https://doi.org/10.1186/s12711-024-00933-3
Access Level:acceso abierto
Palabra clave:636
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oai_identifier_str oai:repositori.irta.cat:20.500.12327/3596
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
title Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
spellingShingle Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
Liu, Junhui
636
title_short Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
title_full Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
title_fullStr Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
title_full_unstemmed Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
title_sort Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigs
dc.creator.none.fl_str_mv Liu, Junhui
Sebastià, Cristina
JOVÉ JUNCÀ, TEODOR
Quintanilla, Raquel
González-Rodríguez, Olga
Passols, Magí
Castelló, Anna
Sánchez, Armand
Ballester Devis, Maria
Folch, Josep M.
author Liu, Junhui
author_facet Liu, Junhui
Sebastià, Cristina
JOVÉ JUNCÀ, TEODOR
Quintanilla, Raquel
González-Rodríguez, Olga
Passols, Magí
Castelló, Anna
Sánchez, Armand
Ballester Devis, Maria
Folch, Josep M.
author_role author
author2 Sebastià, Cristina
JOVÉ JUNCÀ, TEODOR
Quintanilla, Raquel
González-Rodríguez, Olga
Passols, Magí
Castelló, Anna
Sánchez, Armand
Ballester Devis, Maria
Folch, Josep M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Genètica i Millora Animal
dc.subject.none.fl_str_mv 636
topic 636
description Background The composition and distribution of fatty acids (FA) are important factors determining the quality, flavor, and nutrient value of meat. In addition, FAs synthesized in the body participate in energy metabolism and are involved in different regulatory pathways in the form of signaling molecules or by acting as agonist or antagonist ligands of different nuclear receptors. Finally, synthesis and catabolism of FAs affect adaptive immunity by regulating lymphocyte metabolism. The present study performed genome-wide association studies using FA profiles of blood, liver, backfat and muscle from 432 commercial Duroc pigs. Results Twenty-five genomic regions located on 15 Sus scrofa chromosomes (SSC) were detected. Annotation of the quantitative trait locus (QTL) regions identified 49 lipid metabolism-related candidate genes. Among these QTLs, four were identified in more than one tissue. The ratio of C20:4n-6/C20:3n-6 was associated with the region on SSC2 at 7.56–14.26 Mb for backfat, liver, and muscle. Members of the fatty acid desaturase gene cluster (FADS1, FADS2, and FADS3) are the most promising candidate genes in this region. Two QTL regions on SSC14 (103.81–115.64 Mb and 100.91–128.14 Mb) were identified for FA desaturation in backfat and muscle. In addition, two separate regions on SSC9 at 0 – 14.55 Mb and on SSC12 at 0–1.91 Mb were both associated with the same multiple FA traits for backfat, with candidate genes involved in de novo FA synthesis and triacylglycerol (TAG) metabolism, such as DGAT2 and FASN. The ratio C20:0/C18:0 was associated with the region on SSC5 at 64.84–78.32 Mb for backfat. Furthermore, the association of the C16:0 content with the region at 118.92–123.95 Mb on SSC4 was blood specific. Finally, candidate genes involved in de novo lipogenesis regulate T cell differentiation and promote the generation of palmitoleate, an adipokine that alleviates inflammation. Conclusions Several SNPs and candidate genes were associated with lipid metabolism in blood, liver, backfat, and muscle. These results contribute to elucidating the molecular mechanisms implicated in the determination of the FA profile in different pig tissues and can be useful in selection programs that aim to improve health and energy metabolism in pigs.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3596
https://doi.org/10.1186/s12711-024-00933-3
url http://hdl.handle.net/20.500.12327/3596
https://doi.org/10.1186/s12711-024-00933-3
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Genetics Selection Evolution
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C21/ES/FISIOLOGIA MOLECULAR DEL INMUNOMETABOLISMO EN PORCINO: BASES PARA LA SELECCION DE POBLACIONES MAS ROBUSTAS/
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C22/ES/FISIOLOGIA MOLECULAR DEL METABOLISMO ENERGETICO EN PORCINO: BASES PARA LA SELECCION DE LA EFICIENCIA PRODUCTIVA Y CALIDAD DE CARNE/
EC/H2020/817998/EU/The regulatory GENomE of SWine and CHicken: functional annotation during development/GENE-SWitCH
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Identification of genomic regions associated with fatty acid metabolism across blood, liver, backfat and muscle in pigsLiu, JunhuiSebastià, CristinaJOVÉ JUNCÀ, TEODORQuintanilla, RaquelGonzález-Rodríguez, OlgaPassols, MagíCastelló, AnnaSánchez, ArmandBallester Devis, MariaFolch, Josep M.636Background The composition and distribution of fatty acids (FA) are important factors determining the quality, flavor, and nutrient value of meat. In addition, FAs synthesized in the body participate in energy metabolism and are involved in different regulatory pathways in the form of signaling molecules or by acting as agonist or antagonist ligands of different nuclear receptors. Finally, synthesis and catabolism of FAs affect adaptive immunity by regulating lymphocyte metabolism. The present study performed genome-wide association studies using FA profiles of blood, liver, backfat and muscle from 432 commercial Duroc pigs. Results Twenty-five genomic regions located on 15 Sus scrofa chromosomes (SSC) were detected. Annotation of the quantitative trait locus (QTL) regions identified 49 lipid metabolism-related candidate genes. Among these QTLs, four were identified in more than one tissue. The ratio of C20:4n-6/C20:3n-6 was associated with the region on SSC2 at 7.56–14.26 Mb for backfat, liver, and muscle. Members of the fatty acid desaturase gene cluster (FADS1, FADS2, and FADS3) are the most promising candidate genes in this region. Two QTL regions on SSC14 (103.81–115.64 Mb and 100.91–128.14 Mb) were identified for FA desaturation in backfat and muscle. In addition, two separate regions on SSC9 at 0 – 14.55 Mb and on SSC12 at 0–1.91 Mb were both associated with the same multiple FA traits for backfat, with candidate genes involved in de novo FA synthesis and triacylglycerol (TAG) metabolism, such as DGAT2 and FASN. The ratio C20:0/C18:0 was associated with the region on SSC5 at 64.84–78.32 Mb for backfat. Furthermore, the association of the C16:0 content with the region at 118.92–123.95 Mb on SSC4 was blood specific. Finally, candidate genes involved in de novo lipogenesis regulate T cell differentiation and promote the generation of palmitoleate, an adipokine that alleviates inflammation. Conclusions Several SNPs and candidate genes were associated with lipid metabolism in blood, liver, backfat, and muscle. These results contribute to elucidating the molecular mechanisms implicated in the determination of the FA profile in different pig tissues and can be useful in selection programs that aim to improve health and energy metabolism in pigs.This research was financially supported as part of the project METAPIGEN PID2020-112677RB-C21-22 funded by MCIN/AEI/https://doi.org/10.13039/501100011033. We also acknowledge the support from H2020 GENE-SWitCH (grant agreement n° 817998) project and by grant CEX2019-000902-S funded by MCIN/AEI/https://doi.org/10.13039/501100011033. In addition, J. Liu was funded by a Ph.D. fellowship from the China Scholarship Council (CSC), C.Sebastià was funded with a FI-AGAUR PhD grant from the Generalitat de Catalunya (2020FI_B_00225). M.Passols was recipient of grant PRE2018-085350 funded by MCIN/AEI/ https://doi.org/10.13039/501100011033 and by “ESF Investing in your future”, and T. Jové-Juncà with an IRTA fellowship (CPI1221).info:eu-repo/semantics/publishedVersionBioMed CentralProducció AnimalGenètica i Millora Animal202520252024info:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/20.500.12327/3596https://doi.org/10.1186/s12711-024-00933-3reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésGenetics Selection EvolutionMICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C21/ES/FISIOLOGIA MOLECULAR DEL INMUNOMETABOLISMO EN PORCINO: BASES PARA LA SELECCION DE POBLACIONES MAS ROBUSTAS/MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C22/ES/FISIOLOGIA MOLECULAR DEL METABOLISMO ENERGETICO EN PORCINO: BASES PARA LA SELECCION DE LA EFICIENCIA PRODUCTIVA Y CALIDAD DE CARNE/EC/H2020/817998/EU/The regulatory GENomE of SWine and CHicken: functional annotation during development/GENE-SWitCHMICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/35962026-06-16T08:51:17Z
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