Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803

Chemicals and CAS Registry Numbers carbon dioxide 124-38-9, 58561-67-4 glucose 50-99-7, 84778-64-3, 8027-56-3 glucose 1 phosphate adenylyltransferase 9027-71-8 glycogen 9005-79-2 oxygen 7782-44-7 phosphoglucomutase 9001-81-4 Carbon Dioxide Glucose Glucose-1-Phosphate Adenylyltransferase Glycogen Oxy...

Descripción completa

Detalles Bibliográficos
Autores: Ortega-Martínez, Pablo, Nikkanen, Lauri, Wey, Laura T, Florencio, Francisco J, Allahverdiyeva, Yagut, Díaz-Troya, Sandra
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/377042
Acceso en línea:http://hdl.handle.net/10261/377042
https://api.elsevier.com/content/abstract/scopus_id/85192473336
Access Level:acceso abierto
Palabra clave:photosynthesis
glucose
glycogen
metabolism
cyanobacteria
mixotrophy
photomixotrophy
id ES_1f8ecc0a3d763ce18ebf74bfcfca4aee
oai_identifier_str oai:digital.csic.es:10261/377042
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
title Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
spellingShingle Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
Ortega-Martínez, Pablo
photosynthesis
glucose
glycogen
metabolism
cyanobacteria
mixotrophy
photomixotrophy
glycogen
title_short Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
title_full Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
title_fullStr Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
title_full_unstemmed Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
title_sort Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803
dc.creator.none.fl_str_mv Ortega-Martínez, Pablo
Nikkanen, Lauri
Wey, Laura T
Florencio, Francisco J
Allahverdiyeva, Yagut
Díaz-Troya, Sandra
author Ortega-Martínez, Pablo
author_facet Ortega-Martínez, Pablo
Nikkanen, Lauri
Wey, Laura T
Florencio, Francisco J
Allahverdiyeva, Yagut
Díaz-Troya, Sandra
author_role author
author2 Nikkanen, Lauri
Wey, Laura T
Florencio, Francisco J
Allahverdiyeva, Yagut
Díaz-Troya, Sandra
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Junta de Andalucía
Novo Nordisk Foundation
Ministerio de Universidades (España)
University of Turku
Ortega-Martínez, Pablo [0000-0001-5508-5636]
Nikkanen, Lauri [0000-0002-7192-9322]
Wey, Laura T [0000-0003-2345-0699]
Florencio, Francisco J [0000-0002-2068-7861]
Allahverdiyeva, Yagut [0000-0002-9262-1757]
Díaz-Troya, Sandra [0000-0002-5878-9062]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv photosynthesis
glucose
glycogen
metabolism
cyanobacteria
mixotrophy
photomixotrophy
glycogen
topic photosynthesis
glucose
glycogen
metabolism
cyanobacteria
mixotrophy
photomixotrophy
glycogen
description Chemicals and CAS Registry Numbers carbon dioxide 124-38-9, 58561-67-4 glucose 50-99-7, 84778-64-3, 8027-56-3 glucose 1 phosphate adenylyltransferase 9027-71-8 glycogen 9005-79-2 oxygen 7782-44-7 phosphoglucomutase 9001-81-4 Carbon Dioxide Glucose Glucose-1-Phosphate Adenylyltransferase Glycogen Oxygen Phosphoglucomutase Photosystem II Protein Complex
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/377042
https://api.elsevier.com/content/abstract/scopus_id/85192473336
url http://hdl.handle.net/10261/377042
https://api.elsevier.com/content/abstract/scopus_id/85192473336
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104513GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138317NB-I00
FPU18/06580
NNF20OC0064371
https://doi.org/10.1111/nph.19793
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/pdf
dc.publisher.none.fl_str_mv Wiley-Liss
publisher.none.fl_str_mv Wiley-Liss
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404407507976192
spelling Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803Ortega-Martínez, PabloNikkanen, LauriWey, Laura TFlorencio, Francisco JAllahverdiyeva, YagutDíaz-Troya, SandraphotosynthesisglucoseglycogenmetabolismcyanobacteriamixotrophyphotomixotrophyglycogenChemicals and CAS Registry Numbers carbon dioxide 124-38-9, 58561-67-4 glucose 50-99-7, 84778-64-3, 8027-56-3 glucose 1 phosphate adenylyltransferase 9027-71-8 glycogen 9005-79-2 oxygen 7782-44-7 phosphoglucomutase 9001-81-4 Carbon Dioxide Glucose Glucose-1-Phosphate Adenylyltransferase Glycogen Oxygen Phosphoglucomutase Photosystem II Protein ComplexSome cyanobacteria can grow photoautotrophically or photomixotrophically by using simultaneously CO2 and glucose. The switch between these trophic modes and the role of glycogen, their main carbon storage macromolecule, was investigated. We analysed the effect of glucose addition on the physiology, metabolic and photosynthetic state of Synechocystis sp. PCC 6803 and mutants lacking phosphoglucomutase and ADP-glucose pyrophosphorylase, with limitations in glycogen synthesis. Glycogen acted as a metabolic buffer: glucose addition increased growth and glycogen reserves in the wild-type (WT), but arrested growth in the glycogen synthesis mutants. Already 30 min after glucose addition, metabolites from the Calvin-Benson-Bassham cycle and the oxidative pentose phosphate shunt increased threefold more in the glycogen synthesis mutants than the WT. These alterations substantially affected the photosynthetic performance of the glycogen synthesis mutants, as O2 evolution and CO2 uptake were both impaired. We conclude that glycogen synthesis is essential during transitions to photomixotrophy to avoid metabolic imbalance that induces inhibition of electron transfer from PSII and subsequently accumulation of reactive oxygen species, loss of PSII core proteins, and cell death. Our study lays foundations for optimising photomixotrophy-based biotechnologies through understanding the coordination of the crosstalk between photosynthetic electron transport and metabolism.This work was funded by grants PID2019-104513GB-I00 and PID2022-138317NB-I00 both financed by MCIU/AEI/10.13039/501100011033/‘FEDER Una manera de hacer Europa’ and by Junta de Andalucía, Group BIO-0284 to FJF and financially supported by the NordForsk Nordic Center of Excellence ‘NordAqua’ (no. 82845 to YA), the Novo Nordisk Foundation project ‘PhotoCat’ project (no. NNF20OC0064371 to YA). PO-M is a recipient of grant FPU18/06580 from the Ministerio de Universidades and an EMBO Scientific Exchange Grant. We thank Dr Tuomas Huokko and Michal Hubacek for assistance with PO-M's research visit to University of Turku, and Dr Luis López-Maury for critical reading of the manuscript. The photosynthetic electron transport studies (except Chl fluorescence analysis) were conducted in the PhotoSYN Finnish Infrastructure for Photosynthesis Research.Peer reviewedWiley-LissMinisterio de Ciencia, Innovación y Universidades (España)European CommissionJunta de AndalucíaNovo Nordisk FoundationMinisterio de Universidades (España)University of TurkuOrtega-Martínez, Pablo [0000-0001-5508-5636]Nikkanen, Lauri [0000-0002-7192-9322]Wey, Laura T [0000-0003-2345-0699]Florencio, Francisco J [0000-0002-2068-7861]Allahverdiyeva, Yagut [0000-0002-9262-1757]Díaz-Troya, Sandra [0000-0002-5878-9062]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionaplication/pdfhttp://hdl.handle.net/10261/377042https://api.elsevier.com/content/abstract/scopus_id/85192473336reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104513GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138317NB-I00FPU18/06580NNF20OC0064371https://doi.org/10.1111/nph.19793Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3770422026-05-22T06:33:51Z
score 15,811543

Similares en LA Referencia