Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System
18 Páginas.-- 12 Figuras.-- 2 Tablas
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/265618 |
| Acceso en línea: | http://hdl.handle.net/10261/265618 https://api.elsevier.com/content/abstract/scopus_id/85120542132 |
| Access Level: | acceso abierto |
| Palabra clave: | Lipoic acid Lipoyl synthase mitochondrial lipoylation Octanoyltransferase Sunflower |
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Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme SystemMartins-Noguerol, RaquelAcket, SébastienTroncoso-Ponce, M. A.Garcés Mancheño, RafaelThomasset, BrigitteVenegas-Calerón, MónicaSalas, Joaquín J.Martínez-Force, EnriqueMoreno-Pérez, Antonio J.Lipoic acidLipoyl synthasemitochondrial lipoylationOctanoyltransferaseSunflower18 Páginas.-- 12 Figuras.-- 2 TablasLipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of the octanoyltransferase (LIP2, EC 2.3.1.181) and lipoyl synthase (LIP1, EC 2.8.1.8) enzymes. In plants, pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase or glycine decarboxylase are essential complexes that need to be lipoylated. These lipoylated enzymes and complexes are located in the mitochondria, while PDH is also present in plastids where it provides acetyl-CoA for de novo fatty acid biosynthesis. As such, lipoylation of PDH could regulate fatty acid synthesis in both these organelles. In the present work, the sunflower LIP1 and LIP2 genes (HaLIP1m and HaLIP2m) were isolated sequenced, cloned, and characterized, evaluating their putative mitochondrial location. The expression of these genes was studied in different tissues and protein docking was modeled. The genes were also expressed in Escherichia coli and Arabidopsis thaliana, where their impact on fatty acid and glycerolipid composition was assessed. Lipidomic studies in Arabidopsis revealed lipid remodeling in lines overexpressing these enzymes and the involvement of both sunflower proteins in the phenotypes observed is discussed in the light of the results obtained.This work was financed by the PID2020-113134RB-I00/AEI/10.13039/501100011033 project granted by the Spanish State Research Agency within the State Programs for Research and Innovation Oriented to the Challenges of Society.Peer reviewedFrontiers MediaMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/265618https://api.elsevier.com/content/abstract/scopus_id/85120542132reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113134RB-I00Frontiers in plant sciencehttp://dx.doi.org/10.3389/fpls.2021.781917Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2656182026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| title |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| spellingShingle |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System Martins-Noguerol, Raquel Lipoic acid Lipoyl synthase mitochondrial lipoylation Octanoyltransferase Sunflower |
| title_short |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| title_full |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| title_fullStr |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| title_full_unstemmed |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| title_sort |
Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System |
| dc.creator.none.fl_str_mv |
Martins-Noguerol, Raquel Acket, Sébastien Troncoso-Ponce, M. A. Garcés Mancheño, Rafael Thomasset, Brigitte Venegas-Calerón, Mónica Salas, Joaquín J. Martínez-Force, Enrique Moreno-Pérez, Antonio J. |
| author |
Martins-Noguerol, Raquel |
| author_facet |
Martins-Noguerol, Raquel Acket, Sébastien Troncoso-Ponce, M. A. Garcés Mancheño, Rafael Thomasset, Brigitte Venegas-Calerón, Mónica Salas, Joaquín J. Martínez-Force, Enrique Moreno-Pérez, Antonio J. |
| author_role |
author |
| author2 |
Acket, Sébastien Troncoso-Ponce, M. A. Garcés Mancheño, Rafael Thomasset, Brigitte Venegas-Calerón, Mónica Salas, Joaquín J. Martínez-Force, Enrique Moreno-Pérez, Antonio J. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Ciencia, Innovación y Universidades (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Lipoic acid Lipoyl synthase mitochondrial lipoylation Octanoyltransferase Sunflower |
| topic |
Lipoic acid Lipoyl synthase mitochondrial lipoylation Octanoyltransferase Sunflower |
| description |
18 Páginas.-- 12 Figuras.-- 2 Tablas |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/265618 https://api.elsevier.com/content/abstract/scopus_id/85120542132 |
| url |
http://hdl.handle.net/10261/265618 https://api.elsevier.com/content/abstract/scopus_id/85120542132 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113134RB-I00 Frontiers in plant science http://dx.doi.org/10.3389/fpls.2021.781917 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869404404181893120 |
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15.81155 |