Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120

Uptake of 16 amino acids by the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 was characterized with regard to kinetic parameters of transport, intracellular accumulation of the transported amino acids, and sensitivity of the transport process to energy metabolism inhib...

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Autores: Montesinos, María Luz, Herrero Moreno, Antonia, Flores García, Enrique
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1995
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/63107
Acceso en línea:http://hdl.handle.net/11441/63107
Access Level:acceso abierto
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spelling Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120Montesinos, María LuzHerrero Moreno, AntoniaFlores García, EnriqueUptake of 16 amino acids by the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 was characterized with regard to kinetic parameters of transport, intracellular accumulation of the transported amino acids, and sensitivity of the transport process to energy metabolism inhibitors. Mutants resistant to certain toxic analogs of some amino acids were isolated that were impaired in amino acid transport. Results obtained in this study, together with those reported previously (A. Herrero and E. Flores, J. Biol. Chem. 265:3931-3935, 1990), suggest that there are at least five amino acid transport systems in strain PCC 7120: one high- affinity, active system for basic amino acids; one low-affinity, passive system for basic amino acids; two high-affinity, active systems with overlapping, but not identical, specificities for neutral amino acids; and one putative system for acidic amino acids. Some of the amino acid transport mutants were impaired in diazotrophic growth. These mutants were unable to develop a normal percentage of heterocysts and normal nitrogenase activity in response to nitrogen stepdown. Putative roles for the amino acid transport systems in uptake of extracellular amino acids, recapture of amino acids that have leaked from the cells, and intercellular transfer of amino acids in the filaments of Anabaena sp. strain PCC 7120 are discussed.Dirección General de Investigación Científica y Técnica PB90-0114Comisión Interministerial de Ciencia y Tecnología BIO93-0124American Society for MicrobiologyBioquímica Vegetal y Biología MolecularDirección General de Investigación Científica y Técnica (DGICYT). EspañaComisión Interministerial de Ciencia y Tecnología (CICYT). España1995info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/63107reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Bacteriology, 177 (11), 3150-3157.PB90-0114BIO93-0124info:eu-repo/semantics/openAccessoai:idus.us.es:11441/631072026-06-17T12:51:07Z
dc.title.none.fl_str_mv Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
title Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
spellingShingle Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
Montesinos, María Luz
title_short Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
title_full Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
title_fullStr Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
title_full_unstemmed Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
title_sort Amino acid transport systems required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120
dc.creator.none.fl_str_mv Montesinos, María Luz
Herrero Moreno, Antonia
Flores García, Enrique
author Montesinos, María Luz
author_facet Montesinos, María Luz
Herrero Moreno, Antonia
Flores García, Enrique
author_role author
author2 Herrero Moreno, Antonia
Flores García, Enrique
author2_role author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Dirección General de Investigación Científica y Técnica (DGICYT). España
Comisión Interministerial de Ciencia y Tecnología (CICYT). España
description Uptake of 16 amino acids by the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 was characterized with regard to kinetic parameters of transport, intracellular accumulation of the transported amino acids, and sensitivity of the transport process to energy metabolism inhibitors. Mutants resistant to certain toxic analogs of some amino acids were isolated that were impaired in amino acid transport. Results obtained in this study, together with those reported previously (A. Herrero and E. Flores, J. Biol. Chem. 265:3931-3935, 1990), suggest that there are at least five amino acid transport systems in strain PCC 7120: one high- affinity, active system for basic amino acids; one low-affinity, passive system for basic amino acids; two high-affinity, active systems with overlapping, but not identical, specificities for neutral amino acids; and one putative system for acidic amino acids. Some of the amino acid transport mutants were impaired in diazotrophic growth. These mutants were unable to develop a normal percentage of heterocysts and normal nitrogenase activity in response to nitrogen stepdown. Putative roles for the amino acid transport systems in uptake of extracellular amino acids, recapture of amino acids that have leaked from the cells, and intercellular transfer of amino acids in the filaments of Anabaena sp. strain PCC 7120 are discussed.
publishDate 1995
dc.date.none.fl_str_mv 1995
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/63107
url http://hdl.handle.net/11441/63107
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Bacteriology, 177 (11), 3150-3157.
PB90-0114
BIO93-0124
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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