Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability

The keystone of planarian taxonomy traditionally has been the anatomy of the copulatory apparatus. However, many planarian species comprise asexual fissiparous populations, with the fissiparous animals not developing a copulatory apparatus, thus precluding their morphological identification. Incorpo...

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Autores: Leria Florensa, Laia, Vila Farré, Miquel, Álvarez Presas, Marta, Sánchez-Gracia, Alejandro, Rozas Liras, Julio A., Sluys, Ronald, Riutort León, Marta
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/153722
Acesso em linha:https://hdl.handle.net/2445/153722
Access Level:Acceso aberto
Palavra-chave:Planària (Gènere)
Taxonomia (Biologia)
Biologia d'aigua dolça
Planaria (Genus)
Taxonomy (Biology)
Freshwater biology
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spelling Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variabilityLeria Florensa, LaiaVila Farré, MiquelÁlvarez Presas, MartaSánchez-Gracia, AlejandroRozas Liras, Julio A.Sluys, RonaldRiutort León, MartaPlanària (Gènere)Taxonomia (Biologia)Biologia d'aigua dolçaPlanaria (Genus)Taxonomy (Biology)Freshwater biologyThe keystone of planarian taxonomy traditionally has been the anatomy of the copulatory apparatus. However, many planarian species comprise asexual fissiparous populations, with the fissiparous animals not developing a copulatory apparatus, thus precluding their morphological identification. Incorporation of molecular data into planarian systematics has been of great value, not only in the identification of fissiparous individuals but also as an additional source of information for determining species boundaries. Nevertheless, the discrepancy between morphological and molecular data has highlighted the need for extra sources of taxonomic information. Moreover, a recent study has pointed out that fissiparous reproduction may lead to high levels of intraindividual genetic diversity in planarians, which may mislead molecular analyses. In the present study we aim to test a new up-to-date integrative taxonomic procedure for planarians, including intraindividual genetic data and additional sources of taxonomic information, besides morphology and DNA, using Dugesia subtentaculata sensu lato as a model organism, a species with an intricate taxonomic history. First, we used three different methods for molecular species delimitation on single locus datasets, both with and without intraindividual information, for formulating Primary Species Hypotheses (PSHs). Subsequently, Secondary Species Hypotheses (SSHs) were formulated on the basis of three types of information: (1) a coalescent-based species delimitation method applied to multilocus data, (2) morphology of the copulatory apparatus, and (3) karyological metrics. This resulted in the delimitation of four morphologically cryptic species within the nominal species D. subtentaculata. Our results provide evidence that the analysis of intraindividual genetic data is essential for properly developing PSHs in planarians. Our study reveals also that karyological differentiation, rather than morphological differentiation, may play an important role in speciation processes in planarians, thus suggesting that the currently known diversity of the group could be highly underestimated.Elsevier2020202020192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion24 p.application/pdfhttps://hdl.handle.net/2445/153722Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.ympev.2019.05.010Molecular Phylogenetics and Evolution, 2019, vol. 143, p. 106496https://doi.org/10.1016/j.ympev.2019.05.010cc-by-nc-nd (c) Elsevier, 2019http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1537222026-05-29T05:05:01Z
dc.title.none.fl_str_mv Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
title Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
spellingShingle Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
Leria Florensa, Laia
Planària (Gènere)
Taxonomia (Biologia)
Biologia d'aigua dolça
Planaria (Genus)
Taxonomy (Biology)
Freshwater biology
title_short Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
title_full Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
title_fullStr Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
title_full_unstemmed Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
title_sort Cryptic species delineation in freshwater planarians of the genus Dugesia (Platyhelminthes, Tricladida): extreme intraindividual genetic diversity, morphological stasis, and karyological variability
dc.creator.none.fl_str_mv Leria Florensa, Laia
Vila Farré, Miquel
Álvarez Presas, Marta
Sánchez-Gracia, Alejandro
Rozas Liras, Julio A.
Sluys, Ronald
Riutort León, Marta
author Leria Florensa, Laia
author_facet Leria Florensa, Laia
Vila Farré, Miquel
Álvarez Presas, Marta
Sánchez-Gracia, Alejandro
Rozas Liras, Julio A.
Sluys, Ronald
Riutort León, Marta
author_role author
author2 Vila Farré, Miquel
Álvarez Presas, Marta
Sánchez-Gracia, Alejandro
Rozas Liras, Julio A.
Sluys, Ronald
Riutort León, Marta
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Planària (Gènere)
Taxonomia (Biologia)
Biologia d'aigua dolça
Planaria (Genus)
Taxonomy (Biology)
Freshwater biology
topic Planària (Gènere)
Taxonomia (Biologia)
Biologia d'aigua dolça
Planaria (Genus)
Taxonomy (Biology)
Freshwater biology
description The keystone of planarian taxonomy traditionally has been the anatomy of the copulatory apparatus. However, many planarian species comprise asexual fissiparous populations, with the fissiparous animals not developing a copulatory apparatus, thus precluding their morphological identification. Incorporation of molecular data into planarian systematics has been of great value, not only in the identification of fissiparous individuals but also as an additional source of information for determining species boundaries. Nevertheless, the discrepancy between morphological and molecular data has highlighted the need for extra sources of taxonomic information. Moreover, a recent study has pointed out that fissiparous reproduction may lead to high levels of intraindividual genetic diversity in planarians, which may mislead molecular analyses. In the present study we aim to test a new up-to-date integrative taxonomic procedure for planarians, including intraindividual genetic data and additional sources of taxonomic information, besides morphology and DNA, using Dugesia subtentaculata sensu lato as a model organism, a species with an intricate taxonomic history. First, we used three different methods for molecular species delimitation on single locus datasets, both with and without intraindividual information, for formulating Primary Species Hypotheses (PSHs). Subsequently, Secondary Species Hypotheses (SSHs) were formulated on the basis of three types of information: (1) a coalescent-based species delimitation method applied to multilocus data, (2) morphology of the copulatory apparatus, and (3) karyological metrics. This resulted in the delimitation of four morphologically cryptic species within the nominal species D. subtentaculata. Our results provide evidence that the analysis of intraindividual genetic data is essential for properly developing PSHs in planarians. Our study reveals also that karyological differentiation, rather than morphological differentiation, may play an important role in speciation processes in planarians, thus suggesting that the currently known diversity of the group could be highly underestimated.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/153722
url https://hdl.handle.net/2445/153722
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.ympev.2019.05.010
Molecular Phylogenetics and Evolution, 2019, vol. 143, p. 106496
https://doi.org/10.1016/j.ympev.2019.05.010
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier, 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier, 2019
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 24 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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