Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids

Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active si...

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Detalhes bibliográficos
Autores: Adelfinskaya, O., Terrazas Martínez, Montserrat, Froeyen, M., Marlière, P., Nauwelaerts, K., Herdewijn, P.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/179405
Acesso em linha:https://hdl.handle.net/2445/179405
Access Level:acceso abierto
Palavra-chave:ADN
Aminoàcids
Polímers
DNA
Amino acids
Polymers
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repository_id_str
spelling Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acidsAdelfinskaya, O.Terrazas Martínez, MontserratFroeyen, M.Marlière, P.Nauwelaerts, K.Herdewijn, P.ADNAminoàcidsPolímersDNAAmino acidsPolymersSome selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate moiety of deoxyadenosine triphosphate. L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology.Oxford University Press2021202120072021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/179405Articles publicats en revistes (Química Inorgànica i Orgànica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1093/nar/gkm498Nucleic Acids Research, 2007, vol. 35, num. 15, p. 5060-5072https://doi.org/10.1093/nar/gkm498cc-by-nc (c) Adelfinskaya, O. et al., 2007https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1794052026-05-29T05:05:01Z
dc.title.none.fl_str_mv Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
title Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
spellingShingle Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
Adelfinskaya, O.
ADN
Aminoàcids
Polímers
DNA
Amino acids
Polymers
title_short Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
title_full Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
title_fullStr Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
title_full_unstemmed Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
title_sort Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
dc.creator.none.fl_str_mv Adelfinskaya, O.
Terrazas Martínez, Montserrat
Froeyen, M.
Marlière, P.
Nauwelaerts, K.
Herdewijn, P.
author Adelfinskaya, O.
author_facet Adelfinskaya, O.
Terrazas Martínez, Montserrat
Froeyen, M.
Marlière, P.
Nauwelaerts, K.
Herdewijn, P.
author_role author
author2 Terrazas Martínez, Montserrat
Froeyen, M.
Marlière, P.
Nauwelaerts, K.
Herdewijn, P.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ADN
Aminoàcids
Polímers
DNA
Amino acids
Polymers
topic ADN
Aminoàcids
Polímers
DNA
Amino acids
Polymers
description Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate moiety of deoxyadenosine triphosphate. L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology.
publishDate 2007
dc.date.none.fl_str_mv 2007
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179405
url https://hdl.handle.net/2445/179405
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1093/nar/gkm498
Nucleic Acids Research, 2007, vol. 35, num. 15, p. 5060-5072
https://doi.org/10.1093/nar/gkm498
dc.rights.none.fl_str_mv cc-by-nc (c) Adelfinskaya, O. et al., 2007
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Adelfinskaya, O. et al., 2007
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Química Inorgànica i Orgànica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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