Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from...

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Autores: Calero-Bernal, Rafael, Santín, Mónica, Maloney, Jenny G, Martín-Pérez, Manuel, Habela, Miguel Ángel, Fernández-García, José L, Figueiredo, Ana, Nájera, Fernando, Palacios, María J, Mateo, Marta, Balseiro, Ana, Barral, Marta, Lima-Barberoi, José F, Köster, Pamela Carolina, Carmena, David
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/11317
Acceso en línea:http://hdl.handle.net/20.500.12105/11317
Access Level:acceso abierto
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spelling Blastocystis sp. Subtype Diversity in Wild Carnivore Species from SpainCalero-Bernal, RafaelSantín, MónicaMaloney, Jenny GMartín-Pérez, ManuelHabela, Miguel ÁngelFernández-García, José LFigueiredo, AnaNájera, FernandoPalacios, María JMateo, MartaBalseiro, AnaBarral, MartaLima-Barberoi, José FKöster, Pamela CarolinaCarmena, DavidThe occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.WileyInstituto de Salud Carlos IIIMinisterio de Economía y Competitividad (España)Complutense University of Madrid (España)Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)20202020-11-1120202020-03-0120202020-03-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12105/11317reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES PI16CIII 00024open accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/113172026-06-12T12:43:37Z
dc.title.none.fl_str_mv Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
title Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
spellingShingle Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
Calero-Bernal, Rafael
title_short Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
title_full Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
title_fullStr Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
title_full_unstemmed Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
title_sort Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
dc.creator.none.fl_str_mv Calero-Bernal, Rafael
Santín, Mónica
Maloney, Jenny G
Martín-Pérez, Manuel
Habela, Miguel Ángel
Fernández-García, José L
Figueiredo, Ana
Nájera, Fernando
Palacios, María J
Mateo, Marta
Balseiro, Ana
Barral, Marta
Lima-Barberoi, José F
Köster, Pamela Carolina
Carmena, David
author Calero-Bernal, Rafael
author_facet Calero-Bernal, Rafael
Santín, Mónica
Maloney, Jenny G
Martín-Pérez, Manuel
Habela, Miguel Ángel
Fernández-García, José L
Figueiredo, Ana
Nájera, Fernando
Palacios, María J
Mateo, Marta
Balseiro, Ana
Barral, Marta
Lima-Barberoi, José F
Köster, Pamela Carolina
Carmena, David
author_role author
author2 Santín, Mónica
Maloney, Jenny G
Martín-Pérez, Manuel
Habela, Miguel Ángel
Fernández-García, José L
Figueiredo, Ana
Nájera, Fernando
Palacios, María J
Mateo, Marta
Balseiro, Ana
Barral, Marta
Lima-Barberoi, José F
Köster, Pamela Carolina
Carmena, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Ministerio de Economía y Competitividad (España)
Complutense University of Madrid (España)
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
description The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-11-11
2020
2020-03-01
2020
2020-03-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/11317
url http://hdl.handle.net/20.500.12105/11317
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES PI16CIII 00024
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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