T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates

10 pages, 7 figures.-- Journal now known as FEBS Journal.-- Open Access: Content older than 1 year.

Detalles Bibliográficos
Autores: Atencia, Eva Ana, Madrid, Olga, Günther Sillero, María A., Sillero, Antonio
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24355
Acceso en línea:http://hdl.handle.net/10261/24355
Access Level:acceso abierto
Palabra clave:RNA ligase
Dinucleoside polyphosphates
Ap3A
RNA joining
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spelling T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphatesAtencia, Eva AnaMadrid, OlgaGünther Sillero, María A.Sillero, AntonioRNA ligaseDinucleoside polyphosphatesAp3ARNA joining10 pages, 7 figures.-- Journal now known as FEBS Journal.-- Open Access: Content older than 1 year.T4 RNA ligase has been shown to synthesize nucleoside and dinucleoside 5'-polyphosphates by displacement of the AMP from the E-AMP complex with polyphosphates and nucleoside diphosphates and triphosphates. Displacement of the AMP by tripolyphosphate (P3) was concentration dependent, as measured by SDS/PAGE. When the enzyme was incubated in the presence of 0.02 mm[α-32P] ATP, synthesis of labeled Ap4A was observed: ATP was acting as both donor (Km, µm) and acceptor (Km, mm) of AMP from the enzyme. Whereas, as previously known, ATP or dATP (but not other nucleotides) were able to form the E-AMP complex, the specificity of a compound to be acceptor of AMP from the E-AMP complex was very broad, and with Km values between 1 and 2 mm. In the presence of a low concentration (0.02 mm) of [α-32P] ATP (enough to form the E-AMP complex, but only marginally enough to form Ap4A) and 4 mm of the indicated nucleotides or P3, the relative rate of synthesis of the following radioactive (di)nucleotides was observed: Ap4X (from XTP, 100); Ap4dG (from dGTP, 74); Ap4G (from GTP, 49); Ap4dC (from dCTP, 23); Ap4C (from CTP, 9); Ap3A (from ADP, 5); Ap4ddA, (from ddATP, 1); p4A (from P3, 200). The enzyme also synthesized efficiently Ap3A in the presence of 1 mm ATP and 2 mm ADP. The following T4 RNA ligase donors were inhibitors of the synthesis of Ap4G: pCp > pAp > pA2'p.This investigation was supported by grants from the Dirección General de Investigación Científica y Técnica (PM95/13) and Comunidad de Madrid (08.9/0004/1998).Peer reviewedWiley-Blackwell201020101999info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65011026951 bytesapplication/pdfhttp://hdl.handle.net/10261/24355reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1046/j.1432-1327.1999.00338.xinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/243552026-05-22T06:33:51Z
dc.title.none.fl_str_mv T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
title T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
spellingShingle T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
Atencia, Eva Ana
RNA ligase
Dinucleoside polyphosphates
Ap3A
RNA joining
title_short T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
title_full T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
title_fullStr T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
title_full_unstemmed T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
title_sort T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates
dc.creator.none.fl_str_mv Atencia, Eva Ana
Madrid, Olga
Günther Sillero, María A.
Sillero, Antonio
author Atencia, Eva Ana
author_facet Atencia, Eva Ana
Madrid, Olga
Günther Sillero, María A.
Sillero, Antonio
author_role author
author2 Madrid, Olga
Günther Sillero, María A.
Sillero, Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv RNA ligase
Dinucleoside polyphosphates
Ap3A
RNA joining
topic RNA ligase
Dinucleoside polyphosphates
Ap3A
RNA joining
description 10 pages, 7 figures.-- Journal now known as FEBS Journal.-- Open Access: Content older than 1 year.
publishDate 1999
dc.date.none.fl_str_mv 1999
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24355
url http://hdl.handle.net/10261/24355
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1046/j.1432-1327.1999.00338.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1026951 bytes
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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