Protocol for the assessment of mTOR activity in mouse primary hepatocytes.

We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yiel...

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Detalles Bibliográficos
Autores: Plata-Gómez, Ana Belén, Crespo, María, de la Calle Arregui, Celia, de Prado-Rivas, Lucía, Sabio, Guadalupe, Efeyan, Alejo
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/18915
Acceso en línea:http://hdl.handle.net/20.500.12105/18915
Access Level:acceso abierto
Palabra clave:TOR Serine-Threonine Kinases
Animals
Cell Culture Techniques
Cells, Cultured
Hepatocytes
Liver
Mice
Signal Transduction
Descripción
Sumario:We present a protocol for measuring the activity of the mechanistic target of rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as a tool for genetic, pharmacological, metabolomic, and signal transduction procedures. We discuss critical aspects for improving yield, viability, and modulation of the mTOR pathway. This protocol can be adapted to other signaling cascades and is compatible with multiple readouts. For complete details on the use and execution of this protocol, please refer to Ortega-Molina et al. (2021).