Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/296163 |
| Acceso en línea: | http://hdl.handle.net/10261/296163 https://api.elsevier.com/content/abstract/scopus_id/85148075900 |
| Access Level: | acceso abierto |
| Palabra clave: | Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
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Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutantsGutiérrez-Martín, DanielMarquès, MontsePons-Escoda, AlbertVidal, NoemiBruna, JordiRestrepo-Montes, EstebanLópez-Serna, RebecaGarcía-Sayago, FranciscoMajos, CarlesGago-Ferrero, PabloGil-Solsona, RubénSuspect screeningBead beatingHuman biomonitoringHuman tissuesLC-HRMSNon-target screeningLittle is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).This work was supported by Ramón y Cajal fellowship (RYC2019–027913-I, Pablo Gago-Ferrero) from the AEI-MICI. Authors also acknowledge the Spanish Ministry of Science and Innovation through the support received as “Centro de Excelencia Severo Ochoa 2019–2023″. This study is partially supported by a grant from Instituto de Salud Carlos III (ISCIII) (PI20/00360 to AP-E and CM) with support of Bellvitge Biomedical Research Institute (IDIBELL). Co-funded by European Regional Development Fund, ERDF, a way to build Europe. We thank CERCA program / Generalitat de Catalunya for Institutional Support.Peer reviewedElsevierGil-Solsona, Rubén [0000-0002-4269-1036]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/296163https://api.elsevier.com/content/abstract/scopus_id/85148075900reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.mex.2023.102069Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2961632026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| title |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| spellingShingle |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants Gutiérrez-Martín, Daniel Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
| title_short |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| title_full |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| title_fullStr |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| title_full_unstemmed |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| title_sort |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
| dc.creator.none.fl_str_mv |
Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén |
| author |
Gutiérrez-Martín, Daniel |
| author_facet |
Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén |
| author_role |
author |
| author2 |
Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Gil-Solsona, Rubén [0000-0002-4269-1036] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
| topic |
Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
| description |
Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/296163 https://api.elsevier.com/content/abstract/scopus_id/85148075900 |
| url |
http://hdl.handle.net/10261/296163 https://api.elsevier.com/content/abstract/scopus_id/85148075900 |
| dc.language.none.fl_str_mv |
Inglés |
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Inglés |
| dc.relation.none.fl_str_mv |
https://doi.org/10.1016/j.mex.2023.102069 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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15.812429 |