The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms

Lipoprotein lipase (LPL) is responsible for the intravascular catabolism of triglyceride-rich lipoproteins and plays a central role in whole-body energy balance and lipid homeostasis. As such, LPL is subject to tissue-specific regulation in different physiological conditions, but the mechanisms of t...

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Autores: Carulla, Pere, Badia-Villanueva, Míriam, Civit Vives, Sergi, Carrascal Pérez, Montserrat, Abian, Joaquin, Ricart i Jané, David, Llobera i Sande, Miquel, Casanovas Torrequebrada, Albert, López Tejero, M. Dolores
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/218928
Acceso en línea:https://hdl.handle.net/2445/218928
Access Level:acceso abierto
Palabra clave:Teixit adipós
Lipases
Metabolisme dels lípids
Dejuni (Dieta)
Electroforesi
Lipoproteïnes
Adipose tissues
Lipase
Lipid metabolism
Fasting
Electrophoresis
Lipoproteins
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spelling The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoformsCarulla, PereBadia-Villanueva, MíriamCivit Vives, SergiCarrascal Pérez, MontserratAbian, JoaquinRicart i Jané, DavidLlobera i Sande, MiquelCasanovas Torrequebrada, AlbertLópez Tejero, M. DoloresTeixit adipósLipasesMetabolisme dels lípidsDejuni (Dieta)ElectroforesiLipoproteïnesAdipose tissuesLipaseLipid metabolismFastingElectrophoresisLipoproteinsLipoprotein lipase (LPL) is responsible for the intravascular catabolism of triglyceride-rich lipoproteins and plays a central role in whole-body energy balance and lipid homeostasis. As such, LPL is subject to tissue-specific regulation in different physiological conditions, but the mechanisms of this regulation remain incompletely characterized. Previous work revealed that LPL comprises a set of proteoforms with different isoelectric points, but their regulation and functional significance have not been studied thus far. Here we studied the distribution of LPL proteoforms in different rat tissues and their regulation under physiological conditions. First, analysis by two-dimensional electrophoresis and Western blot showed different patterns of LPL proteoforms (i.e., different pI or relative abundance of LPL proteoforms) in different rat tissues under basal conditions, which could be related to the tissue-specific regulation of the enzyme. Next, the comparison of LPL proteoforms from heart and brown adipose tissue between adults and 15-day-old rat pups, two conditions with minimal regulation of LPL in these tissues, yielded virtually the same tissue-specific patterns of LPL proteoforms. In contrast, the pronounced downregulation of LPL activity observed in white adipose tissue during fasting is accompanied by a prominent reconfiguration of the LPL proteoform pattern. Furthermore, refeeding reverts this downregulation of LPL activity and restores the pattern of LPL proteoforms in this tissue. Importantly, this reversible proteoform-specific regulation during fasting and refeeding indicates that LPL proteoforms are functionally diverse. Further investigation of potential differences in the functional properties of LPL proteoforms showed that all proteoforms exhibit lipolytic activity and have similar heparin-binding affinity, although other functional aspects remain to be investigated. Overall, this study demonstrates the ubiquity, differential distribution and specific regulation of LPL proteoforms in rat tissues and underscores the need to consider the existence of LPL proteoforms for a complete understanding of LPL regulation under physiological conditions.Frontiers Media2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/218928Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fphys.2023.1271149Frontiers in Physiology, 2023, vol. 14, p. 1-12https://doi.org/10.3389/fphys.2023.1271149cc-by (c) Carulla, P. et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2189282026-05-29T05:05:01Z
dc.title.none.fl_str_mv The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
title The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
spellingShingle The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
Carulla, Pere
Teixit adipós
Lipases
Metabolisme dels lípids
Dejuni (Dieta)
Electroforesi
Lipoproteïnes
Adipose tissues
Lipase
Lipid metabolism
Fasting
Electrophoresis
Lipoproteins
title_short The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
title_full The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
title_fullStr The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
title_full_unstemmed The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
title_sort The response to fasting and refeeding reveals functional regulation of lipoprotein lipase proteoforms
dc.creator.none.fl_str_mv Carulla, Pere
Badia-Villanueva, Míriam
Civit Vives, Sergi
Carrascal Pérez, Montserrat
Abian, Joaquin
Ricart i Jané, David
Llobera i Sande, Miquel
Casanovas Torrequebrada, Albert
López Tejero, M. Dolores
author Carulla, Pere
author_facet Carulla, Pere
Badia-Villanueva, Míriam
Civit Vives, Sergi
Carrascal Pérez, Montserrat
Abian, Joaquin
Ricart i Jané, David
Llobera i Sande, Miquel
Casanovas Torrequebrada, Albert
López Tejero, M. Dolores
author_role author
author2 Badia-Villanueva, Míriam
Civit Vives, Sergi
Carrascal Pérez, Montserrat
Abian, Joaquin
Ricart i Jané, David
Llobera i Sande, Miquel
Casanovas Torrequebrada, Albert
López Tejero, M. Dolores
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Teixit adipós
Lipases
Metabolisme dels lípids
Dejuni (Dieta)
Electroforesi
Lipoproteïnes
Adipose tissues
Lipase
Lipid metabolism
Fasting
Electrophoresis
Lipoproteins
topic Teixit adipós
Lipases
Metabolisme dels lípids
Dejuni (Dieta)
Electroforesi
Lipoproteïnes
Adipose tissues
Lipase
Lipid metabolism
Fasting
Electrophoresis
Lipoproteins
description Lipoprotein lipase (LPL) is responsible for the intravascular catabolism of triglyceride-rich lipoproteins and plays a central role in whole-body energy balance and lipid homeostasis. As such, LPL is subject to tissue-specific regulation in different physiological conditions, but the mechanisms of this regulation remain incompletely characterized. Previous work revealed that LPL comprises a set of proteoforms with different isoelectric points, but their regulation and functional significance have not been studied thus far. Here we studied the distribution of LPL proteoforms in different rat tissues and their regulation under physiological conditions. First, analysis by two-dimensional electrophoresis and Western blot showed different patterns of LPL proteoforms (i.e., different pI or relative abundance of LPL proteoforms) in different rat tissues under basal conditions, which could be related to the tissue-specific regulation of the enzyme. Next, the comparison of LPL proteoforms from heart and brown adipose tissue between adults and 15-day-old rat pups, two conditions with minimal regulation of LPL in these tissues, yielded virtually the same tissue-specific patterns of LPL proteoforms. In contrast, the pronounced downregulation of LPL activity observed in white adipose tissue during fasting is accompanied by a prominent reconfiguration of the LPL proteoform pattern. Furthermore, refeeding reverts this downregulation of LPL activity and restores the pattern of LPL proteoforms in this tissue. Importantly, this reversible proteoform-specific regulation during fasting and refeeding indicates that LPL proteoforms are functionally diverse. Further investigation of potential differences in the functional properties of LPL proteoforms showed that all proteoforms exhibit lipolytic activity and have similar heparin-binding affinity, although other functional aspects remain to be investigated. Overall, this study demonstrates the ubiquity, differential distribution and specific regulation of LPL proteoforms in rat tissues and underscores the need to consider the existence of LPL proteoforms for a complete understanding of LPL regulation under physiological conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/218928
url https://hdl.handle.net/2445/218928
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fphys.2023.1271149
Frontiers in Physiology, 2023, vol. 14, p. 1-12
https://doi.org/10.3389/fphys.2023.1271149
dc.rights.none.fl_str_mv cc-by (c) Carulla, P. et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Carulla, P. et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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