Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up

MicroRNAs (miRNA) are major regulators of intercellular communication and key players in the pathophysiology of cardiovascular disease. This study aimed to determine the miRNA fingerprint in a cohort of 53 patients with acute myocardial infarction (AMI) with non-ST-segment elevation (NSTEMI) relativ...

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Autores: Mompeón, Ana, Perez Cremades, Daniel, Paes, Ana Belén, Sanchis, Juan, Ortega Paz, Luis, Andrea, Rut, Brugaletta, Salvatore, Sabate, Manel, Novella, Susana, Dantas, Ana Paula, Hermenegildo, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/201581
Acceso en línea:https://hdl.handle.net/2445/201581
Access Level:acceso abierto
Palabra clave:Micro RNAs
Infart de miocardi
Sèrum
Marcadors bioquímics
Endoteli
MicroRNAs
Myocardial infarction
Serum
Biochemical markers
Endothelium
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spelling Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-UpMompeón, AnaPerez Cremades, DanielPaes, Ana BelénSanchis, JuanOrtega Paz, LuisAndrea, RutBrugaletta, SalvatoreSabate, ManelNovella, SusanaDantas, Ana PaulaHermenegildo, CarlosMicro RNAsInfart de miocardiSèrumMarcadors bioquímicsEndoteliMicroRNAsMyocardial infarctionSerumBiochemical markersEndotheliumMicroRNAs (miRNA) are major regulators of intercellular communication and key players in the pathophysiology of cardiovascular disease. This study aimed to determine the miRNA fingerprint in a cohort of 53 patients with acute myocardial infarction (AMI) with non-ST-segment elevation (NSTEMI) relative to miRNA expression in healthy controls (n = 51). miRNA expression was initially profiled by miRNA array in the serum of patients undergoing cardiac catheterization during NSTEMI (n = 8) and 1 year past the event (follow-up, n = 8) and validated in the entire cohort. In total, 58 miRNAs were differentially expressed during AMI (p < 0.05), while 36 were modified at follow-up (Fisher's exact test: p = 0.0138). Enrichment analyses revealed differential regulation of biological processes by miRNA at each specific time point (AMI vs. follow-up). During AMI, the miRNA profile was associated mainly with processes involved in vascular development. However, 1 year after AMI, changes in miRNA expression were partially related to the regulation of cardiac tissue morphogenesis. Linear correlation analysis of miRNA with serum levels of cytokines and chemokines revealed that let-7g-5p, let-7e-5p, and miR-26a-5p expression was inversely associated with serum levels of pro-inflammatory cytokines TNF-α, and the chemokines MCP-3 and MDC. Transient transfection of human endothelial cells (HUVEC) with let-7e-5p inhibitor or mimic demonstrated a key role for this miRNA in endothelial function regulation in terms of cell adhesion and angiogenesis capacity. HUVEC transfected with let-7e-5p mimic showed a 20% increase in adhesion capacity, whereas transfection with let-7e-5p inhibitor increased the number of tube-like structures. This study pinpoints circulating miRNA expression fingerprint in NSTEMI patients, specific to the acute event and changes at 1-year follow-up. Additionally, given its involvement in modulating endothelial cell function and vascularization, altered let-7e-5p expression may constitute a therapeutic biomarker and target for ischemic heart disease.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/201581Articles publicats en revistes (Medicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/cells11111823Cells, 2022, vol. 11, num. 11, p. 1823https://doi.org/10.3390/cells11111823cc-by (c) Mompeón, Ana et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2015812026-05-27T06:46:51Z
dc.title.none.fl_str_mv Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
title Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
spellingShingle Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
Mompeón, Ana
Micro RNAs
Infart de miocardi
Sèrum
Marcadors bioquímics
Endoteli
MicroRNAs
Myocardial infarction
Serum
Biochemical markers
Endothelium
title_short Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
title_full Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
title_fullStr Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
title_full_unstemmed Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
title_sort Circulating miRNA Fingerprint and Endothelial Function in Myocardial Infarction: Comparison at Acute Event and One-Year Follow-Up
dc.creator.none.fl_str_mv Mompeón, Ana
Perez Cremades, Daniel
Paes, Ana Belén
Sanchis, Juan
Ortega Paz, Luis
Andrea, Rut
Brugaletta, Salvatore
Sabate, Manel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author Mompeón, Ana
author_facet Mompeón, Ana
Perez Cremades, Daniel
Paes, Ana Belén
Sanchis, Juan
Ortega Paz, Luis
Andrea, Rut
Brugaletta, Salvatore
Sabate, Manel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author_role author
author2 Perez Cremades, Daniel
Paes, Ana Belén
Sanchis, Juan
Ortega Paz, Luis
Andrea, Rut
Brugaletta, Salvatore
Sabate, Manel
Novella, Susana
Dantas, Ana Paula
Hermenegildo, Carlos
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Micro RNAs
Infart de miocardi
Sèrum
Marcadors bioquímics
Endoteli
MicroRNAs
Myocardial infarction
Serum
Biochemical markers
Endothelium
topic Micro RNAs
Infart de miocardi
Sèrum
Marcadors bioquímics
Endoteli
MicroRNAs
Myocardial infarction
Serum
Biochemical markers
Endothelium
description MicroRNAs (miRNA) are major regulators of intercellular communication and key players in the pathophysiology of cardiovascular disease. This study aimed to determine the miRNA fingerprint in a cohort of 53 patients with acute myocardial infarction (AMI) with non-ST-segment elevation (NSTEMI) relative to miRNA expression in healthy controls (n = 51). miRNA expression was initially profiled by miRNA array in the serum of patients undergoing cardiac catheterization during NSTEMI (n = 8) and 1 year past the event (follow-up, n = 8) and validated in the entire cohort. In total, 58 miRNAs were differentially expressed during AMI (p < 0.05), while 36 were modified at follow-up (Fisher's exact test: p = 0.0138). Enrichment analyses revealed differential regulation of biological processes by miRNA at each specific time point (AMI vs. follow-up). During AMI, the miRNA profile was associated mainly with processes involved in vascular development. However, 1 year after AMI, changes in miRNA expression were partially related to the regulation of cardiac tissue morphogenesis. Linear correlation analysis of miRNA with serum levels of cytokines and chemokines revealed that let-7g-5p, let-7e-5p, and miR-26a-5p expression was inversely associated with serum levels of pro-inflammatory cytokines TNF-α, and the chemokines MCP-3 and MDC. Transient transfection of human endothelial cells (HUVEC) with let-7e-5p inhibitor or mimic demonstrated a key role for this miRNA in endothelial function regulation in terms of cell adhesion and angiogenesis capacity. HUVEC transfected with let-7e-5p mimic showed a 20% increase in adhesion capacity, whereas transfection with let-7e-5p inhibitor increased the number of tube-like structures. This study pinpoints circulating miRNA expression fingerprint in NSTEMI patients, specific to the acute event and changes at 1-year follow-up. Additionally, given its involvement in modulating endothelial cell function and vascularization, altered let-7e-5p expression may constitute a therapeutic biomarker and target for ischemic heart disease.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/201581
url https://hdl.handle.net/2445/201581
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cells11111823
Cells, 2022, vol. 11, num. 11, p. 1823
https://doi.org/10.3390/cells11111823
dc.rights.none.fl_str_mv cc-by (c) Mompeón, Ana et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Mompeón, Ana et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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